Target of Interest
Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Application
图: 1 / 4
C14orf179 Antibody (PA5-144009) in ICC/IF
Immunocytochemistry analysis of C14orf179 in T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in C14orf179 Polyclonal antibody (Product # PA5-144009) using a dilution of 5 μg/mL. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and inc... View More
产品信息
PA5-144009
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human IFT43 recombinant protein (Position: M1-T208).
偶联物
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_3075223
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U-87MG whole cell, human T-47D whole cell, human MCF-7 whole cell, human PC-3 whole cell, human A549 whole cell, human Jurkat whole cell, human A431 whole cell, rat testis tissue, rat kidney tissue, rat liver tissue, mouse testis tissue, mouse kidney tissue, mouse liver tissue. ICC/IF: T-47D cell. Flow: Caco-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
IFT43, encoded by C14ORF179, is a subunit of the intraflagellar transport complex A (IFT-A) of primary cilia. The IFT-A is implicated in retrograde ciliary transport along axonemal microtubules. Defects in subunits of IFT-A has been associated with skeletal ciliopathies, including Sensenbrenner syndrome, Jeune syndrome, and Ellis-van-Creveld syndrome. Mutations in C14ORF179 has been linked to Sensenbrenner syndrome. (21378380).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: IFT complex A subunit; intraflagellar transport 43 homolog; Intraflagellar transport protein 43 homolog
基因别名: 1700019E19Rik; C14orf179; CED3; IFT43; R75064; RGD1307392
UniProt ID: (Human) Q96FT9, (Mouse) Q9DA69
Entrez Gene ID: (Human) 112752, (Mouse) 76411, (Rat) 299209
