Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), FITC, eBioscience™

CCL2 (MCP-1) Antibody in Flow Cytometry (Flow)

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CCL2 (MCP-1) Antibody (11-7096-81) in Flow

Staining of 1-day LPS-stimulated human peripheral blood cells with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (open histogram) or 0.5 µg of Anti-CCL2 (MCP-1) FITC (filled histogram). Cells in the monocyte gate were used for analysis.

CCL2 (MCP-1) Antibody in Immunocytochemistry (ICC/IF)

CCL2 (MCP-1) Antibody in Immunohistochemistry (IHC)

CCL2 (MCP-1) Antibody in Neutralization (Neu)

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产品信息

11-7096-81

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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中和实验 (Neu)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2H5

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465399

产品详细信息

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported:The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5 antibody (Product # 16-7096-81) in functional assays).

Applications Tested: Has been tested by intracellular staining and flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

Immunity

Pekayvaz K,Gold C,Hoseinpour P,Engel A,Martinez-Navarro A,Eivers L,Coletti R,Joppich M,Dionísio F,Kaiser R,Tomas L,Janjic A,Knott M,Mehari F,Polewka V,Kirschner M,Boda A,Nicolai L,Schulz H,Titova A,Kilani B,Lorenz M,Fingerle-Rowson G,Bucala R,Enard W,Zimmer R,Weber C,Libby P,Schulz C,Massberg S,Stark K

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 11-7096-81) in Neutralization

Tue Oct 10 00:00:00 EDT 2023

Astrocytic interleukin-3 programs microglia and limits Alzheimer's disease.

Nature

McAlpine CS,Park J,Griciuc A,Kim E,Choi SH,Iwamoto Y,Kiss MG,Christie KA,Vinegoni C,Poller WC,Mindur JE,Chan CT,He S,Janssen H,Wong LP,Downey J,Singh S,Anzai A,Kahles F,Jorfi M,Feruglio PF,Sadreyev RI,Weissleder R,Kleinstiver BP,Nahrendorf M,Tanzi RE,Swirski FK

11-7096-81 was used in Flow cytometry/Cell sorting to identify IL-3 as a key mediator of astrocyte-microglia cross-talk and a node for therapeutic intervention in Alzheimer's disease.

Thu Jul 01 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 11-7096-81) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 11-7096-81) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Molecular medicine reports

Shen C,Lie P,Miao T,Yu M,Lu Q,Feng T,Li J,Zu T,Liu X,Li H

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 11-7096-81) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

生物信息学

蛋白别名: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2

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基因别名: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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功能

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding cytokine intercellular signal molecule

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