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CCR9 Antibody (MA5-33122) in ICC/IF
Immunofluorescent analysis of CCR9 in HepG2 cells using a CCR9 monoclonal antibody (Product # MA5-33122) at a dilution of 1:33. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.
产品信息
MA5-33122
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4G2
抗原
A synthesized peptide derived from human CCR9
偶联物
形式
Liquid
浓度
1.5 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2811938
靶标信息
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; CDw199; CDw199 antigen; chemokine (C-C motif) receptor 9; Chemokine C-C receptor 10; G protein-coupled receptor 28; G-protein coupled receptor 28; GPR-9-6
基因别名: A130091K22Rik; CC-CKR-9; CCR9; CDw199; Cmkbr10; GPR-9-6; GPR28
UniProt ID: (Human) P51686, (Mouse) Q9WUT7
Entrez Gene ID: (Human) 10803, (Mouse) 12769
