Invitrogen

CD101 Monoclonal Antibody (Moushi101), PE-Cyanine7, eBioscience™

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CD101 Antibody (25-1011-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) FITC (Product # 11-5931-82) and 0.125 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.125 µg of Anti-Mouse CD101 PE-Cyanine7 (right). Cells in the granulocyte gate were used for analysis.

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产品信息

25-1011-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

Moushi101

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573378

产品详细信息

Description: The monoclonal antibody moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains). CD101 is a transmembrane glycoprotein containing 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation. CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells and activated T cells. In addition, CD101 is found on a subset of CD25+Foxp3+ T regulatory cells that showed higher suppressive properties in vitro and in vivo.

Applications Reported: This Moushi101 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Moushi101 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD101 also known as V7 antigen, a member of the Ig superfamily. CD101 is a disulfide linked homodimer of unknown function. Expression of CD101 is found on monocytes, granulocytes and dendritic cells (Langerhan-like cells HLA-DR, CD1a, CD1c). In addition expression on T lymphocytes is important for cell activation. CD101+ CD28+ cells are very responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can increase proliferation thereby suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit the T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Recently it has been demonstrated that mouse CD101 is found on a subpopulation of regulatory T cells (CD4, CD25, Foxp3 positive) that have high suppressor activity. Expression of CD101 on human PBMCs shows staining of about 30% of the Foxp3 positive cells. Studies have not confirmed higher suppressor activity in the human CD101 population.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Eosinophils protect against pulmonary hypertension through 14-HDHA and 17-HDHA.

The European respiratory journal

Shu T,Zhang J,Zhou Y,Chen Z,Li J,Tang Q,Lei W,Xing Y,Wang J,Wang C

25-1011-82 was used in Flow cytometry/Cell sorting to show that in PH development without external inflammatory stimulus, peripheral blood exhibits a low EOS level, in which EOS play a protective role through suppressing perivascular inflammation and maintaining PASMC homeostasis via 14/17-HDHA.

Wed Mar 01 00:00:00 EST 2023

CXCL10 chemokine regulates heterogeneity of the CD8⁺ T cell response and viral set point during chronic infection.

Immunity

Ozga AJ,Chow MT,Lopes ME,Servis RL,Di Pilato M,Dehio P,Lian J,Mempel TR,Luster AD

25-1011-82 was used in Flow cytometry/Cell sorting to find that the chemokine receptor CXCR3 is highly expressed on viral-specific stem-like CD8+ T cells and that one of its ligands, CXCL10, regulates the persistence and heterogeneity of responding CD8+ T cells in spleens of mice chronically infected with lymphocytic choriomeningitis virus.

Tue Jan 11 00:00:00 EST 2022

Antigen-driven EGR2 expression is required for exhausted CD8⁺ T cell stability and maintenance.

Nature communications

Wagle MV,Vervoort SJ,Kelly MJ,Van Der Byl W,Peters TJ,Martin BP,Martelotto LG,Nüssing S,Ramsbottom KM,Torpy JR,Knight D,Reading S,Thia K,Miosge LA,Howard DR,Gloury R,Gabriel SS,Utzschneider DT,Oliaro J,Powell JD,Luciani F,Trapani JA,Johnstone RW,Kallies A,Goodnow CC,Parish IA

25-1011-82 was used in Flow cytometry/Cell sorting to show that persistent antigen recognition drives higher EGR2 expression within exhausted versus effector CD8+T cells.

Thu May 13 00:00:00 EDT 2021

Antigen-driven EGR2 expression is required for exhausted CD8⁺ T cell stability and maintenance.

Nature communications

25-1011-82 was used in Flow cytometry/Cell sorting to show that persistent antigen recognition drives higher EGR2 expression within exhausted versus effector CD8+T cells.

Thu May 13 00:00:00 EDT 2021

Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4⁺ T Cells.

Immunity

Śledzińska A,Vila de Mucha M,Bergerhoff K,Hotblack A,Demane DF,Ghorani E,Akarca AU,Marzolini MAV,Solomon I,Vargas FA,Pule M,Ono M,Seddon B,Kassiotis G,Ariyan CE,Korn T,Marafioti T,Lord GM,Stauss H,Jenner RG,Peggs KS,Quezada SA

25-1011 was used in Flow cytometry/Cell sorting to underscore the role of Treg cells, IL-2, and Blimp-1 in controlling the differentiation of cytotoxic CD4+ T cells and offer a pathway to enhancement of anti-tumor activity through their manipulation.

Tue Jan 14 00:00:00 EST 2020

Proliferating Transitory T Cells with an Effector-like Transcriptional Signature Emerge from PD-1⁺ Stem-like CD8⁺ T Cells during Chronic Infection.

Immunity

Hudson WH,Gensheimer J,Hashimoto M,Wieland A,Valanparambil RM,Li P,Lin JX,Konieczny BT,Im SJ,Freeman GJ,Leonard WJ,Kissick HT,Ahmed R

Tue Dec 17 00:00:00 EST 2019

Epithelial NOTCH Signaling Rewires the Tumor Microenvironment of Colorectal Cancer to Drive Poor-Prognosis Subtypes and Metastasis.

Cancer cell

Jackstadt R,van Hooff SR,Leach JD,Cortes-Lavaud X,Lohuis JO,Ridgway RA,Wouters VM,Roper J,Kendall TJ,Roxburgh CS,Horgan PG,Nixon C,Nourse C,Gunzer M,Clark W,Hedley A,Yilmaz OH,Rashid M,Bailey P,Biankin AV,Campbell AD,Adams DJ,Barry ST,Steele CW,Medema JP,Sansom OJ

Published figure using CD101 monoclonal antibody (Product # 25-1011-82) in Flow Cytometry

Mon Sep 16 00:00:00 EDT 2019

生物信息学

蛋白别名: CD101; EWI immunoglobulin subfamily member EWI-101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IgSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; RP11-27K13.2

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基因别名: Cd101; EWI-101; Gm1016; Gm734; Igsf2

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UniProt ID: (Mouse) A8E0Y8

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Entrez Gene ID: (Mouse) 630146

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功能

molecular_function immunoglobulin receptor superfamily

参与通路

positive regulation of myeloid leukocyte differentiation