Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), FITC, eBioscience™

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

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CD103 (Integrin alpha E) Antibody (11-1038-42) in Flow

Staining of 3-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD103 (Integrin alpha E) FITC (purple histogram). Total viable cells were used for analysis.

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产品信息

11-1038-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (2 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

B-Ly7

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10598799

产品详细信息

Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis.

Applications Tested: This B-Ly7 antibody has been pre-titrated and tested by flow cytometric analysis of 2-3 day PHA-activated human peripheral blood cells. This can be used at 5 µL (2 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1038-42) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.

Scientific reports

Feyaerts D,Benner M,van Cranenbroek B,van der Heijden OWH,Joosten I,van der Molen RG

11-1038 was used in Flow cytometry/Cell sorting to immunophenotype lymphocytes from endometrium, term decidua parietalis, and peripheral blood mononuclear cells for direct comparison.

Tue Jun 06 00:00:00 EDT 2017

CD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits.

PloS one

Schrøder M,Melum GR,Landsverk OJ,Bujko A,Yaqub S,Gran E,Aamodt H,Bækkevold ES,Jahnsen FL,Richter L

11-1038 was used in Flow cytometry/Cell sorting to demonstrate that a widely-used commercial CD1c+ dendritic cell isolation kit isolates two phenotypically and functionally different CD1c+ cell populations.

Thu Jul 13 00:00:00 EDT 2017

Celiac Disease-Related Inflammation Is Marked by Reduction of Nkp44/Nkp46-Double Positive Natural Killer Cells.

PloS one

Marafini I,Monteleone I,Di Fusco D,Sedda S,Cupi ML,Fina D,Paoluzi AO,Pallone F,Monteleone G

11-1038 was used in Flow cytometry/Cell sorting to investigate the nature of disease-related inflammation in coeliacs, with regard to their NK cells.

Mon Jul 10 00:00:00 EDT 2017

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 11-1038-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Prenatally acquired vitamin A deficiency alters innate immune responses to human rotavirus in a gnotobiotic pig model.

Journal of immunology (Baltimore, Md. : 1950)

Vlasova AN,Chattha KS,Kandasamy S,Siegismund CS,Saif LJ

11-1038 was used in Flow cytometry/Cell sorting to examine how prenatally acquired vitamin A deficiency modulates innate immune responses and human rotavirus (HRV) vaccine efficacy in a gnotobiotic piglet model of HRV diarrhoea.

Wed May 01 00:00:00 EDT 2013

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

11-1038 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

生物信息学

蛋白别名: antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; integrin alpha E; Integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); Intregrin alpha E2; MGC141996; Mucosal lymphocyte 1 antigen

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基因别名: CD103; HUMINAE; ITGAE

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UniProt ID: (Human) P38570

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Entrez Gene ID: (Human) 3682

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功能

metal ion binding integrin

参与通路

cell adhesion integrin-mediated signaling pathway extracellular matrix organization