Invitrogen

CD103 (Integrin alpha E) Monoclonal Antibody (B-Ly7), eBioscience™

CD103 (Integrin alpha E) Antibody in Flow Cytometry (Flow)

图: 1 / 5

CD103 (Integrin alpha E) Antibody (14-1038-82) in Flow

Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total viable cells were used for analysis.

Product Image Gallery

产品信息

14-1038-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

- -

免疫组化（冰冻） (IHC (F))

Assay-Dependent

流式细胞分析 (Flow)

0.5 µg/test

查看9篇已发表文章 9篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

B-Ly7

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_467412

产品详细信息

Description: The B-Ly7 monoclonal antibody reacts with human CD103, the alpha E integrin. CD103 non-covalently associates with integrin beta 7. CD103 is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103 is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103. Epithelial cell antigen, E-cadherin, binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium.

Applications Reported: B-Ly7 has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The B-Ly7 antibody has been tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells.

Nature communications

Duhen R,Ballesteros-Merino C,Frye AK,Tran E,Rajamanickam V,Chang SC,Koguchi Y,Bifulco CB,Bernard B,Leidner RS,Curti BD,Fox BA,Urba WJ,Bell RB,Weinberg AD

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 14-1038-82) in Flow Cytometry

Tue Feb 16 00:00:00 EST 2021

Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors.

Nature communications

Duhen T,Duhen R,Montler R,Moses J,Moudgil T,de Miranda NF,Goodall CP,Blair TC,Fox BA,McDermott JE,Chang SC,Grunkemeier G,Leidner R,Bell RB,Weinberg AD

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 14-1038-82) in Flow Cytometry

Fri Jul 13 00:00:00 EDT 2018

Inflammatory cues enhance TGFβ activation by distinct subsets of human intestinal dendritic cells via integrin αvβ8.

Mucosal immunology

Fenton TM,Kelly A,Shuttleworth EE,Smedley C,Atakilit A,Powrie F,Campbell S,Nishimura SL,Sheppard D,Levison S,Worthington JJ,Lehtinen MJ,Travis MA

14-1038 was used in Flow cytometry/Cell sorting to show that a key activator of TGFΒ, integrin αvΒ8, is highly expressed on human intestinal dendritic cells, specifically on the CD1c+ but not the CD141+ intestinal DC subset.

Mon May 01 00:00:00 EDT 2017

Optimization of mass cytometry sample cryopreservation after staining.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Sumatoh HR,Teng KW,Cheng Y,Newell EW

14103882 was used in flow cytometry to demonstrate that freezing already-stained samples suspended in 10% DMSO in FBS is practical and efficient way to preserve already-stained samples for mass cytometry assessment

Sun Jan 01 00:00:00 EST 2017

Tissue Distribution of Memory T and B Cells in Rhesus Monkeys following Influenza A Infection.

Journal of immunology (Baltimore, Md. : 1950)

Pichyangkul S,Yongvanitchit K,Limsalakpetch A,Kum-Arb U,Im-Erbsin R,Boonnak K,Thitithayanont A,Jongkaewwattana A,Wiboon-ut S,Mongkolsirichaikul D,Mahanonda R,Spring M,Chuang I,Mason CJ,Saunders DL

14-1038 was used in Flow cytometry/Cell sorting to identify a subset of influenza-specific lung memory T-cells characterized as tissue-resident memory T-cells in rhesus monkeys.

Sun Nov 01 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD103 (Integrin alpha E) monoclonal antibody (Product # 14-1038-82) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques.

Journal of immunology (Baltimore, Md. : 1950)

Canary LA,Vinton CL,Morcock DR,Pierce JB,Estes JD,Brenchley JM,Klatt NR

14-1038 was used in Flow cytometry/Cell sorting to determine whether pre-existing GI-associated mucosal damage affects the progression of AIDS following HIV infection.

Fri Mar 15 00:00:00 EDT 2013

DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues.

Blood

Poulin LF,Reyal Y,Uronen-Hansson H,Schraml BU,Sancho D,Murphy KM,Håkansson UK,Moita LF,Agace WW,Bonnet D,Reis e Sousa C

14-1038 was used in Flow cytometry/Cell sorting to investigate the presence of unique markers in human dendritic cells, showing that DNGR-1 is a specific marker of mouse and human dendritic cells in lymphoid and nonlymphoid tissues.

Thu Jun 21 00:00:00 EDT 2012

Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.

The Journal of experimental medicine

Poulin LF,Salio M,Griessinger E,Anjos-Afonso F,Craciun L,Chen JL,Keller AM,Joffre O,Zelenay S,Nye E,Le Moine A,Faure F,Donckier V,Sancho D,Cerundolo V,Bonnet D,Reis e Sousa C

14-1038 was used in Flow cytometry/Cell sorting to investigate a population of human DCs that expresses DNGR-1 and high levels of BDCA3.

Mon Jun 07 00:00:00 EDT 2010

生物信息学

蛋白别名: antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; integrin alpha E; Integrin alpha-E; Integrin alpha-IEL; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); Intregrin alpha E2; MGC141996; Mucosal lymphocyte 1 antigen

查看更多收起

基因别名: CD103; HUMINAE; ITGAE

查看更多收起

UniProt ID: (Human) P38570

查看更多收起

Entrez Gene ID: (Human) 3682

查看更多收起

功能

metal ion binding integrin

参与通路

cell adhesion integrin-mediated signaling pathway extracellular matrix organization