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CD107b (LAMP-2) Antibody (MA5-45170) in WB
Western Blot analysis of Human, Mouse HEK293 and 3T3NIH cell lysates showing detection of ~100-110 kDa LAMP2 protein. Lane 1: MW ladder. Lane 2: Human HEK293 lysate (20 µg). Lane 3: Mouse 3T3NIH lysate (10 µg). Blocking: 5% milk + TBST for 1 hour at RT. Samples were incubated with LAMP2 monoclonal antibody (Product # MA5-45170) at 1:500 for 1 hour at RT, followed by HRP Goat Anti-Rat at 1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~100-110 kDa.
产品信息
MA5-45170
产品规格
种属反应
Human,
Mouse,
Rabbit
宿主/亚型
Rat
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
GL2A7
抗原
Purified preparation of mouse liver lysosomal membranes
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 581mM DMSO, 123mM ethanolamine
内含物
no preservative
保存条件
4° C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2931624
产品详细信息
1 µg/mL of MA5-45170 was sufficient for detection of LAMP2 in 20 µg of rat liver microsomes by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 100-110kDa.
靶标信息
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon’s disease).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LGP-96; LGP-B; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; RP23-193O17.2
基因别名: CD107b; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; LAMP2; LAMPB; LGP-B; LGP110; Mac3
UniProt ID: (Human) P13473, (Mouse) P17047
Entrez Gene ID: (Rabbit) 100350946, (Human) 3920, (Mouse) 16784
