Invitrogen

CD115 (c-fms) Monoclonal Antibody (12-3A3-1B10), Super Bright™ 436, eBioscience™

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

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CD115 (c-fms) Antibody (62-1159-42) in Flow

Normal human peripheral blood cells were stained with CD11b Monoclonal Antibody, FITC (Product # 11-0118-42) and Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or CD115 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

62-1159-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

12-3A3-1B10

偶联物

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

激发/发射光谱

413/431 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2688206

产品详细信息

Description: CD115 or Colony-Stimulating Factor-1 Receptor (CSF-1R) is expressed on cells committed to the monocyte lineage and osteoclasts. CSF-1R is a disulfide-linked homodimer, which upon binding of its ligand, CSF-1, undergoes tyrosine autophosphorylation and subsequently, phosphorylates other membrane-proximal downstream targets which results in cytoskeletal remodeling, gene transcription and protein translation. CSF-1R activation promotes the survival, proliferation and differentiation of mononuclear phagocytes and the spreading and motility of macrophages. In osteoclasts, CSF-1R synergizes with RANKL to regulate the differentiation of mononuclear phagocytes to osteoclasts.

Applications Reported: This 12-3A3-1B10 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 12-3A3-1B10 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Macrophage-Colony-Stimulating Factor Receptor Enhances Prostate Cancer Cell Growth and Aggressiveness In Vitro and In Vivo and Increases Osteopontin Expression.

International journal of molecular sciences

Mougel A,Adriaenssens E,Guyot B,Tian L,Gobert S,Chassat T,Persoons P,Hannebique D,Bauderlique-Le Roy H,Vicogne J,Le Bourhis X,Bourette RP

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1159-42) in Flow Cytometry

Fri Dec 16 00:00:00 EST 2022

生物信息学

蛋白别名: CD115; CD115 antigen; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; FMS proto-oncogene; kinase CSFR; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; OTTHUMP00000224093; Proto-oncogene c-Fms

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基因别名: C-FMS; CD115; CSF-1R; CSF1R; CSFR; FIM2; FMS; HDLS; M-CSF-R

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UniProt ID: (Human) P07333

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Entrez Gene ID: (Human) 1436

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功能

macrophage colony-stimulating factor receptor activity ATP binding protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

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