Invitrogen

CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), PE, eBioscience™

图: 1 / 1

CD122 Antibody (12-1222-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse NK1-1 APC (Product # 17-5941-82) and 0.06 µg of Rat IgG2b K Isotype Control PE (Product # 12-4031-82) (left) or 0.06 µg of Anti-Mouse CD122 PE (right). Total viable cells were used for analysis.

Product Image Gallery

产品信息

12-1222-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

TM-b1 (TM-beta1)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465836

产品详细信息

Description: The TM-b1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain (IL-2R beta). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-b1 antibody.

Applications Reported: The TM-b1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Directed evolution of super-secreted variants from phage-displayed human Interleukin-2.

Scientific reports

Rojas G,Carmenate T,Santo-Tomás JF,Valiente PA,Becker M,Pérez-Riverón A,Tundidor Y,Ortiz Y,Fernandez de Cossio-Diaz J,Graça L,Dübel S,León K

12-1222 was used in Flow cytometry/Cell sorting to improve developability of the growing family of IL-2-derived immunotherapeutic agents, which could have a broader impact on the engineering of structurally related four-alpha-helix bundle cytokines.

Mon Jan 28 00:00:00 EST 2019

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

12-1222 was used in Flow cytometry/Cell sorting to provide genetic evidence supporting that NKG2A and the inhibitory members of Ly49 family receptors synergize to regulate NK cell education.

Fri Nov 01 00:00:00 EDT 2019

Restricted Expression of the Thymoproteasome Is Required for Thymic Selection and Peripheral Homeostasis of CD8⁺ T Cells.

Cell reports

Tomaru U,Konno S,Miyajima S,Kimoto R,Onodera M,Kiuchi S,Murata S,Ishizu A,Kasahara M

12-1222 was used in Flow cytometry/Cell sorting to find that thymoproteasomes are essential for positive selection but that the subsequent change in peptide repertoire in the medulla is also crucial for thymic selection and that β5t-derived peptide must be confined to the thymus to avoid autoimmunity in peripheral tissues.

Tue Jan 15 00:00:00 EST 2019

A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion.

Immunity

Xia P,Wang S,Ye B,Du Y,Li C,Xiong Z,Qu Y,Fan Z

12-1222 was used in Flow cytometry/Cell sorting to study circular RNA-mediated maintenance of hematopoeitic stem cell homeostasis.

Tue Apr 17 00:00:00 EDT 2018

Lack of FcRn Impairs Natural Killer Cell Development and Functions in the Tumor Microenvironment.

Frontiers in immunology

Castaneda DC,Dhommée C,Baranek T,Dalloneau E,Lajoie L,Valayer A,Arnoult C,Demattéi MV,Fouquenet D,Parent C,Heuzé-Vourc'h N,Gouilleux-Gruart V

12-1222 was used in Flow cytometry/Cell sorting to elucidate the consequences of neonatal Fc receptor down-regulation in the anti-tumour immune response.

Mon Oct 21 00:00:00 EDT 2019

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

12-1222 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

IFN-γ-producing and IL-17-producing γδ T cells differentiate at distinct developmental stages in murine fetal thymus.

Journal of immunology (Baltimore, Md. : 1950)

Shibata K,Yamada H,Nakamura M,Hatano S,Katsuragi Y,Kominami R,Yoshikai Y

12-1222 was used in Flow cytometry/Cell sorting to compare the functions of γδ T cells derived from double-negative 2 and double-negative 3 cells in the murine fetal thymus.

Sat Mar 01 00:00:00 EST 2014

Cell-to-cell variability analysis dissects the plasticity of signaling of common γ chain cytokines in T cells.

Science signaling

Cotari JW,Voisinne G,Dar OE,Karabacak V,Altan-Bonnet G

12-1222 was used in Flow cytometry/Cell sorting to investigate the effects of IL-2Rα on the sensitivity of primary mouse T lymphocytes to cytokines that signal through receptors that have the common γ chain.

Tue Mar 12 00:00:00 EDT 2013

IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.

Blood

Rubinstein MP,Lind NA,Purton JF,Filippou P,Best JA,McGhee PA,Surh CD,Goldrath AW

12-1222 was used in Flow cytometry/Cell sorting to study IL-7 and IL-15 regulation of CD8+ T-cell subsets during contraction of the immune response.

Sat Nov 01 00:00:00 EDT 2008

Unique receptor repertoire in mouse uterine NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Yadi H,Burke S,Madeja Z,Hemberger M,Moffett A,Colucci F

12-1222-82 was used in Flow Cytometry to define the receptor repertoire and phenotype of uterine NK cells at mid-gestation in mice.

Sat Nov 01 00:00:00 EDT 2008

生物信息学

蛋白别名: CD122; High affinity IL-2 receptor subunit beta; IL-15 receptor beta chain; IL-2 receptor beta chain; IL-2 receptor subunit beta; IL-2/15 receptor-beta; IL-2R subunit beta; IL-2RB; Interleukin-2 receptor subunit beta; p70-75

查看更多收起

基因别名: CD122; IL-15Rbeta; Il-2/15Rbeta; Il-2Rbeta; IL15Rbeta; Il2rb; p70

查看更多收起

UniProt ID: (Mouse) P16297

查看更多收起

Entrez Gene ID: (Mouse) 16185

查看更多收起

功能

cytokine receptor activity interleukin-2 receptor activity interleukin-2 binding transmembrane signal receptor

参与通路

cytokine-mediated signaling pathway natural killer cell activation interleukin-2-mediated signaling pathway negative regulation of apoptotic process