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Invitrogen
CD137 (4-1BB) Monoclonal Antibody (17B5), Functional Grade, eBioscience™
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CD137 (4-1BB) Antibody (16-1371-85) in Flow
Oncoimmunology 2021 -
Figure 1. T-cell activation by professional APCs can lead to distinct immune checkpoint expression on CD8 + T cells . Splenocytes were prepared from the spleens of OT-1 mice and separated into T cells (CD8+) and B-cells (CD19+) using MACS. DC (CD11c+) were prepared from the spleens of Flt3 ligand-treated B6 mice. T cells were stimulated with a control peptide (No Stim), the SIINFEKL peptide alone (No APC), ... View More
产品信息
16-1371-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Syrian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
17B5
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_469069
产品详细信息
Description: The 17B5 monoclonal antibody reacts with mouse CD137, also known as 4-1BB, a member of the TNFR superfamily. CD137 is expressed as monomers, dimers, and tetramers on the surface of activated T cells around day 4-6 after activation through the TCR complex. Interaction of CD137 with its ligand, 4-1BBL, is reported to deliver a costimulatory signal important in the T-APC interaction. Recent data suggests CD317 is expressed on Tregs and may play a role in their proliferation. The monoclonal antibody 17B5 has strong antagonistic effect in in vitro functional assays.
Applications Reported: This 17B5 antibody has been reported for use in flow cytometric analysis and functional assays.
Applications Tested: The 17B5 antibody has been tested by flow cytometric analysis of activated mouse splenocytes cultures. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 4 1 BB; 4-1BB ligand receptor; 41BB; CD137; CD137 antigen; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T-cell antigen 4-1BB; Tumor necrosis factor receptor superfamily member 9
基因别名: 4-1BB; A930040I11Rik; AA408498; AI325004; Cd137; CDw137; ILA; Ly63; Tnfrsf9
UniProt ID: (Mouse) P20334
Entrez Gene ID: (Mouse) 21942
