Invitrogen

CD14 Monoclonal Antibody (61D3), Biotin, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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50 篇参考文献

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说明书

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说明书

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CD14 Antibody (13-0149-82) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD16 PE and CD14 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD14 clone 61D3 stains monocytes (orange) and granulocytes (blue), but not lymphocytes (pink).

CD14 Antibody in Immunocytochemistry (ICC/IF)

CD14 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD14 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD14 Antibody — Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD16 PE and CD14 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD14 clone 61D3 stains monocytes (orange) and granulocytes (blue), but not lymphocytes (pink). {RE}

产品信息

13-0149-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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中和实验 (Neu)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

61D3

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466373

产品详细信息

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.

Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients.

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Fri Mar 10 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

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Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

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Heliangin acts as a covalent ligand of RPS2 that disrupts pre-rRNA metabolic processes in NPM1-mutated acute myeloid leukemia.

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Early macrophage response to obesity encompasses Interferon Regulatory Factor 5 regulated mitochondrial architecture remodelling.

Nature communications

Orliaguet L,Ejlalmanesh T,Humbert A,Ballaire R,Diedisheim M,Julla JB,Chokr D,Cuenco J,Michieletto J,Charbit J,Lindén D,Boucher J,Potier C,Hamimi A,Lemoine S,Blugeon C,Legoix P,Lameiras S,Baudrin LG,Baulande S,Soprani A,Castelli FA,Fenaille F,Riveline JP,Dalmas E,Rieusset J,Gautier JF,Venteclef N,Alzaid F

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A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

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Thu Sep 22 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

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Exposure of a specific pleioform of multifunctional glyceraldehyde 3-phosphate dehydrogenase initiates CD14-dependent clearance of apoptotic cells.

Cell death & disease

Chaudhary S,Patidar A,Dhiman A,Chaubey GK,Dilawari R,Talukdar S,Modanwal R,Raje M

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Neutralization

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The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

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Tue Sep 01 00:00:00 EDT 2020

Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi strains unveil a role for fibroblasts in neutrophil recruitment and activation.

PLoS pathogens

Oliveira AER,Pereira MCA,Belew AT,Ferreira LRP,Pereira LMN,Neves EGA,Nunes MDCP,Burleigh BA,Dutra WO,El-Sayed NM,Gazzinelli RT,Teixeira SMR

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

A functional antibody cross-reactive to both human and murine cytotoxic T-lymphocyte-associated protein 4 via binding to an N-glycosylation epitope.

mAbs

Li D,Li J,Chu H,Wang Z

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Fri Feb 12 00:00:00 EST 2021

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

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Tue Oct 06 00:00:00 EDT 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

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Fri Nov 12 00:00:00 EST 2021

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

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Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

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Chemerin enhances the adhesion and migration of human endothelial progenitor cells and increases lipid accumulation in mice with atherosclerosis.

Lipids in health and disease

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Sun Sep 20 00:00:00 EDT 2020

NLRP3 Inflammasome Activation in Hemodialysis and Hypertensive Patients with Intact Kidney Function.

Toxins

Ulrich C,Wildgrube S,Fiedler R,Seibert E,Kneser L,Fick S,Schäfer C,Markau S,Trojanowicz B,Girndt M

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Mon Oct 26 00:00:00 EDT 2020

LILRB3 (ILT5) is a myeloid cell checkpoint that elicits profound immunomodulation.

JCI insight

Yeboah M,Papagregoriou C,Jones DC,Chan HTC,Hu G,McPartlan JS,Schiött T,Mattson U,Mockridge CI,Tornberg UC,Hambe B,Ljungars A,Mattsson M,Tews I,Glennie MJ,Thirdborough SM,Trowsdale J,Frendeus B,Chen J,Cragg MS,Roghanian A

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NLRP3 inflammasome expression in peripheral blood monocytes of coronary heart disease patients and its modulation by rosuvastatin.

Molecular medicine reports

Zhu J,Wu S,Hu S,Li H,Li M,Geng X,Wang H

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Thu Aug 01 00:00:00 EDT 2019

Characterizing the Role of Monocytes in T Cell Cancer Immunotherapy Using a 3D Microfluidic Model.

Frontiers in immunology

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AURKA Suppresses Leukemic THP-1 Cell Differentiation through Inhibition of the KDM6B Pathway.

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Thu May 31 00:00:00 EDT 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

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Mon Oct 01 00:00:00 EDT 2018

Impaired efferocytosis by monocytes in multiple myeloma.

Oncology letters

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Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

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Cellular metabolism constrains innate immune responses in early human ontogeny.

Nature communications

Kan B,Michalski C,Fu H,Au HHT,Lee K,Marchant EA,Cheng MF,Anderson-Baucum E,Aharoni-Simon M,Tilley P,Mirmira RG,Ross CJ,Luciani DS,Jan E,Lavoie PM

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Fri Nov 16 00:00:00 EST 2018

The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.

Cell death & disease

Ong SM,Hadadi E,Dang TM,Yeap WH,Tan CT,Ng TP,Larbi A,Wong SC

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

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Systemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

Cell

Lim AI,Li Y,Lopez-Lastra S,Stadhouders R,Paul F,Casrouge A,Serafini N,Puel A,Bustamante J,Surace L,Masse-Ranson G,David E,Strick-Marchand H,Le Bourhis L,Cocchi R,Topazio D,Graziano P,Muscarella LA,Rogge L,Norel X,Sallenave JM,Allez M,Graf T,Hendriks RW,Casanova JL,Amit I,Yssel H,Di Santo JP

13-0149 was used in Flow cytometry/Cell sorting to identify circulating and tissue ILC precursors in humans that fail to express the transcription factors and cytokine outputs of mature ILCs but have these signature loci in an epigenetically poised configuration.

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A therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.

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Toll-like receptor 3 activation selectively reverses HIV latency in microglial cells.

Retrovirology

Alvarez-Carbonell D,Garcia-Mesa Y,Milne S,Das B,Dobrowolski C,Rojas R,Karn J

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Immunofluorescence

Mon Feb 06 00:00:00 EST 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Fri Dec 02 00:00:00 EST 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Fri Dec 02 00:00:00 EST 2016

STK4 regulates TLR pathways and protects against chronic inflammation-related hepatocellular carcinoma.

The Journal of clinical investigation

Li W,Xiao J,Zhou X,Xu M,Hu C,Xu X,Lu Y,Liu C,Xue S,Nie L,Zhang H,Li Z,Zhang Y,Ji F,Hui L,Tao W,Wei B,Wang H

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

CD1a+ survivin+ dendritic cell infiltration in dermal lesions of systemic sclerosis.

Arthritis research & therapy

Mokuda S,Miyazaki T,Ubara Y,Kanno M,Sugiyama E,Takasugi K,Masumoto J

13-0149 was used in Magnetic cell separation to investigate the expression and function of survivin in systemic sclerosis.

Wed Sep 30 00:00:00 EDT 2015

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies.

Blood cancer journal

Kim SY,Theunissen JW,Balibalos J,Liao-Chan S,Babcock MC,Wong T,Cairns B,Gonzalez D,van der Horst EH,Perez M,Levashova Z,Chinn L,D'Alessio JA,Flory M,Bermudez A,Jackson DY,Ha E,Monteon J,Bruhns MF,Chen G,Migone TS

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Fri May 29 00:00:00 EDT 2015

The role of transforming growth factor β signaling in fibroblast-like synoviocytes from patients with oligoarticular juvenile idiopathic arthritis: dysregulation of transforming growth factor β signaling, including overexpression of bone morphogenetic protein 4, may lead to a chondrocyte phenotype and may contribute to bony hypertrophy.

Arthritis & rheumatology (Hoboken, N.J.)

Brescia AC,Simonds MM,McCahan SM,Fawcett PT,Rose CD

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Immunofluorescence

Thu May 01 00:00:00 EDT 2014

Human mesenchymal stem cells possess different biological characteristics but do not change their therapeutic potential when cultured in serum free medium.

Stem cell research & therapy

Wang Y,Wu H,Yang Z,Chi Y,Meng L,Mao A,Yan S,Hu S,Zhang J,Zhang Y,Yu W,Ma Y,Li T,Cheng Y,Wang Y,Wang S,Liu J,Han J,Li C,Liu L,Xu J,Han ZB,Han ZC

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Dec 04 00:00:00 EST 2014

Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.

PloS one

Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.

PloS one

Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Jun 25 00:00:00 EDT 2015

Noncanonical dendritic cell differentiation and survival driven by a bacteremic pathogen.

Journal of leukocyte biology

Miles B,Scisci E,Carrion J,Sabino GJ,Genco CA,Cutler CW

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Induction of autophagy is essential for monocyte-macrophage differentiation.

Blood

Zhang Y,Morgan MJ,Chen K,Choksi S,Liu ZG

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Thu Mar 22 00:00:00 EDT 2012

Negative regulation of JAK2 by H3K9 methyltransferase G9a in leukemia.

Molecular and cellular biology

Son HJ,Kim JY,Hahn Y,Seo SB

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2012

Ulcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids.

PloS one

Suárez J,Romero-Zerbo Y,Márquez L,Rivera P,Iglesias M,Bermúdez-Silva FJ,Andreu M,Rodríguez de Fonseca F

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Immunofluorescence

Thu Dec 13 00:00:00 EST 2012

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Ro60-associated single-stranded RNA links inflammation with fetal cardiac fibrosis via ligation of TLRs: a novel pathway to autoimmune-associated heart block.

Journal of immunology (Baltimore, Md. : 1950)

Clancy RM,Alvarez D,Komissarova E,Barrat FJ,Swartz J,Buyon JP

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Mon Feb 15 00:00:00 EST 2010

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD14 monoclonal antibody (Product # 13-0149-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

生物信息学

蛋白别名: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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基因别名: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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功能

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

参与通路