Invitrogen

CD140b (PDGFRB) Monoclonal Antibody (APB5), PE-Cyanine7, eBioscience™

CD140b (PDGFRB) Antibody in Flow Cytometry (Flow)

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CD140b (PDGFRB) Antibody (25-1402-82) in Flow

NIH/3T3 cells were stained with 0.5 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.5 µg of CD140b (PDGFRB) Monoclonal Antibody, PE-Cyanine7 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

CD140b (PDGFRB) Antibody in Immunocytochemistry (ICC/IF)

CD140b (PDGFRB) Antibody in Immunohistochemistry (IHC)

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产品信息

25-1402-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1.0 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

APB5

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848306

产品详细信息

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.

Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

靶标信息

PDGFRB is a receptor tyrosine kinase and has been implicated in atherogenesis and in cell transformation by the SIS oncogene. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB (CD140b) to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Type H vessel/platelet-derived growth factor receptor β⁺ perivascular cell disintegration is involved in vascular injury and bone loss in radiation-induced bone damage.

Cell proliferation

Li J,Chen X,Ren L,Chen X,Wu T,Wang Y,Ren X,Cheng B,Xia J

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration.

Developmental cell

Hwang PY,Mathur J,Cao Y,Almeida J,Ye J,Morikis V,Cornish D,Clarke M,Stewart SA,Pathak A,Longmore GD

25-1402-82 was used in Flow Cytometry to highlight how a subset of leader cells interact with the microenvironment to direct collective migration.

Mon Jan 09 00:00:00 EST 2023

Cellular dormancy in minimal residual disease following targeted therapy.

Breast cancer research : BCR

Ruth JR,Pant DK,Pan TC,Seidel HE,Baksh SC,Keister BA,Singh R,Sterner CJ,Bakewell SJ,Moody SE,Belka GK,Chodosh LA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Distinct Fibroblast Lineages Give Rise to NG2+ Pericyte Populations in Mouse Skin Development and Repair.

Frontiers in cell and developmental biology

Goss G,Rognoni E,Salameti V,Watt FM

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Wed Mar 02 00:00:00 EST 2022

Generation and characterization of a Myh6-driven Cre knockin mouse line.

Transgenic research

Huang X,Yan L,Kou S,Meng J,Lu Z,Lin CP,Liu C,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Immunocytochemistry

Wed Dec 01 00:00:00 EST 2021

Direct contribution of skeletal muscle mesenchymal progenitors to bone repair.

Nature communications

Julien A,Kanagalingam A,Martínez-Sarrà E,Megret J,Luka M,Ménager M,Relaix F,Colnot C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Immunohistochemistry

Mon May 17 00:00:00 EDT 2021

Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.

FEBS open bio

Lee LL,Khakoo AY,Chintalgattu V

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Pulmonary pericytes regulate lung morphogenesis.

Nature communications

Kato K,Diéguez-Hurtado R,Park DY,Hong SP,Kato-Azuma S,Adams S,Stehling M,Trappmann B,Wrana JL,Koh GY,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri Jun 22 00:00:00 EDT 2018

Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.

Developmental cell

Naiman N,Fujioka K,Fujino M,Valerius MT,Potter SS,McMahon AP,Kobayashi A

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Immunocytochemistry

Mon May 22 00:00:00 EDT 2017

Laminin differentially regulates the stemness of type I and type II pericytes.

Stem cell research & therapy

Gautam J,Nirwane A,Yao Y

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Tue Feb 07 00:00:00 EST 2017

Neonatal pancreatic pericytes support β-cell proliferation.

Molecular metabolism

Epshtein A,Rachi E,Sakhneny L,Mizrachi S,Baer D,Landsman L

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Sun Oct 01 00:00:00 EDT 2017

Age-dependent modulation of vascular niches for haematopoietic stem cells.

Nature

Kusumbe AP,Ramasamy SK,Itkin T,Mäe MA,Langen UH,Betsholtz C,Lapidot T,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Thu Apr 21 00:00:00 EDT 2016

Paracrine Wnt1 Drives Interstitial Fibrosis without Inflammation by Tubulointerstitial Cross-Talk.

Journal of the American Society of Nephrology : JASN

Maarouf OH,Aravamudhan A,Rangarajan D,Kusaba T,Zhang V,Welborn J,Gauvin D,Hou X,Kramann R,Humphreys BD

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Analysis of the brain mural cell transcriptome.

Scientific reports

He L,Vanlandewijck M,Raschperger E,Andaloussi Mäe M,Jung B,Lebouvier T,Ando K,Hofmann J,Keller A,Betsholtz C

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

Endothelial cells are progenitors of cardiac pericytes and vascular smooth muscle cells.

Nature communications

Chen Q,Zhang H,Liu Y,Adams S,Eilken H,Stehling M,Corada M,Dejana E,Zhou B,Adams RH

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri Aug 12 00:00:00 EDT 2016

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle.

The Journal of clinical investigation

Quattrocelli M,Swinnen M,Giacomazzi G,Camps J,Barthélemy I,Ceccarelli G,Caluwé E,Grosemans H,Thorrez L,Pelizzo G,Muijtjens M,Verfaillie CM,Blot S,Janssens S,Sampaolesi M

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Regional and stage-specific effects of prospectively purified vascular cells on the adult V-SVZ neural stem cell lineage.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Crouch EE,Liu C,Silva-Vargas V,Doetsch F

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2015

Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy.

PloS one

Mendel TA,Clabough EB,Kao DS,Demidova-Rice TN,Durham JT,Zotter BC,Seaman SA,Cronk SM,Rakoczy EP,Katz AJ,Herman IM,Peirce SM,Yates PA

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Fri Jan 10 00:00:00 EST 2014

Differential contribution to neuroendocrine tumorigenesis of parallel egfr signaling in cancer cells and pericytes.

Genes & cancer

Nolan-Stevaux O,Truitt MC,Pahler JC,Olson P,Guinto C,Lee DC,Hanahan D

Published figure using CD140b (PDGFRB) monoclonal antibody (Product # 25-1402-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2010

生物信息学

蛋白别名: Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b; CD140b antigen; PDGF beta chain; PDGF Receptor beta; PDGF-R-beta; PDGFR-1; PDGFR-beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1

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基因别名: AI528809; CD140b; Pdgfr; PDGFR-1; Pdgfr1; Pdgfrb

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UniProt ID: (Mouse) P05622

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Entrez Gene ID: (Mouse) 18596

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功能