Invitrogen

CD150 Monoclonal Antibody (9D1), PE, eBioscience™

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CD150 Antibody (12-1501-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG1 kappa Isotype Control PE (Product # 12-4301-82) (left) or 0.25 µg of Anti-Mouse CD150 PE (right). Total viable cells were used for analysis.

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产品信息

12-1501-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

9D1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465873

产品详细信息

Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (Product # 12-1502).

Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily, and is involved in T cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane bound form, the soluble form and the cytoplasmic isoforms of SLAM. It is suggested that signalling through homophilic SLAM-SLAM binding during B to B and B to T cell interactions enhances the expansion and differentiation of activated B cells. Reports suggest that the extracellular domain of CD150 is the receptor for the measles virus and acts as a co-activator on T cells and B cells. Moreover, CD150 is thought to interact with SH2D1A and with PTPN11 via its cytoplasmic domain. Mutations CD150 gene may be associated with X-linked lympho-proliferative disease (XLP).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation.

Cancer discovery

Chen S,Vedula RS,Cuevas-Navarro A,Lu B,Hogg SJ,Wang E,Benbarche S,Knorr K,Kim WJ,Stanley RF,Cho H,Erickson C,Singer M,Cui D,Tittley S,Durham BH,Pavletich TS,Fiala E,Walsh MF,Inoue D,Monette S,Taylor J,Rosen N,McCormick F,Lindsley RC,Castel P,Abdel-Wahab O

12-1501-82 was used in Flow cytometry/Cell sorting to reveal proteolysis of non-canonical RAS proteins as novel regulators of HSC self-renewal, define the function of RIT1 and LZTR1 mutations in leukemia, and identify means to overcome drug resistance due to LZTR1 downregulation.

Wed Oct 05 00:00:00 EDT 2022

Establishment of a fluorescent reporter of RNA-polymerase II activity to identify dormant cells.

Nature communications

Freter R,Falletta P,Omrani O,Rasa M,Herbert K,Annunziata F,Minetti A,Krepelova A,Adam L,Käppel S,Rüdiger T,Wang ZQ,Goding CR,Neri F

12-1501-82 was used in Flow Cytometry to identify a dormant OSCARhigh cell population in the small intestine.

Thu Jun 03 00:00:00 EDT 2021

Coordinated alterations in RNA splicing and epigenetic regulation drive leukaemogenesis.

Nature

Yoshimi A,Lin KT,Wiseman DH,Rahman MA,Pastore A,Wang B,Lee SC,Micol JB,Zhang XJ,de Botton S,Penard-Lacronique V,Stein EM,Cho H,Miles RE,Inoue D,Albrecht TR,Somervaille TCP,Batta K,Amaral F,Simeoni F,Wilks DP,Cargo C,Intlekofer AM,Levine RL,Dvinge H,Bradley RK,Wagner EJ,Krainer AR,Abdel-Wahab O

12-1501 was used in Flow cytometry/Cell sorting to study the mutations in IDH2 and SRSF2 that together promote leukaemogenesis through coordinated effects on the epigenome and RNA splicing.

Tue Oct 01 00:00:00 EDT 2019

Synthetic Lethal and Convergent Biological Effects of Cancer-Associated Spliceosomal Gene Mutations.

Cancer cell

Lee SC,North K,Kim E,Jang E,Obeng E,Lu SX,Liu B,Inoue D,Yoshimi A,Ki M,Yeo M,Zhang XJ,Kim MK,Cho H,Chung YR,Taylor J,Durham BH,Kim YJ,Pastore A,Monette S,Palacino J,Seiler M,Buonamici S,Smith PG,Ebert BL,Bradley RK,Abdel-Wahab O

12-1501 was used in Flow cytometry/Cell sorting to identify shared consequences of splicing-factor mutations and the basis for their mutual exclusivity.

Mon Aug 13 00:00:00 EDT 2018

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD150 monoclonal antibody (Product # 12-1501-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

NF-Y is necessary for hematopoietic stem cell proliferation and survival.

Blood

Bungartz G,Land H,Scadden DT,Emerson SG

12-1501 was used in Flow cytometry/Cell sorting to show that the trimeric transcription factor NF-Y is critical for the survival of cycling, but not quiescent hematopoietic stem cells.