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Invitrogen

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience™

5个已发表图片

11 篇参考文献

查看其他68个CD154 (CD40 Ligand)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
已发表图片 (5)

Application

CD154 (CD40 Ligand) Antibody in Flow Cytometry (Flow)

图: 1 / 6

CD154 (CD40 Ligand) Antibody (17-1541-82) in Flow

T cells sorted from BALB/c mouse splenocytes using the MagniSort™ Mouse T cell Enrichment Kit (Product # 8804-6820-74) were stimulated overnight with the Cell Stimulation Cocktail (Product # 00-4970-03) and then stained with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and 0.125 µg of Armenian Hamster IgG Isotype Control, APC (Product # 17-4888-82) (left) or 0.125 µg of CD154 Monoclonal Antibody, AP... View More

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产品信息

17-1541-82

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看10篇已发表文章 10篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

MR1

偶联物

APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_795823

产品详细信息

Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle.

For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1.

Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Strength of tonic T cell receptor signaling instructs T follicular helper cell-fate decisions.

Nature immunology

Bartleson JM,Viehmann Milam AA,Donermeyer DL,Horvath S,Xia Y,Egawa T,Allen PM

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 17-1541-82) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2020

Senescence-associated secretory phenotype promotes chronic ocular graft-vs-host disease in mice and humans.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yamane M,Sato S,Shimizu E,Shibata S,Hayano M,Yaguchi T,Kamijuku H,Ogawa M,Suzuki T,Mukai S,Shimmura S,Okano H,Takeuchi T,Kawakami Y,Ogawa Y,Tsubota K

17-1541-82 was used in Immunohistochemistry (Frozen) to elucidate an additional pathway of chronic ocular chronic graft-vs-host disease.

Sat Aug 01 00:00:00 UTC 2020

A Thpok-Directed Transcriptional Circuitry Promotes Bcl6 and Maf Expression to Orchestrate T Follicular Helper Differentiation.

Immunity

Vacchio MS,Ciucci T,Gao Y,Watanabe M,Balmaceno-Criss M,McGinty MT,Huang A,Xiao Q,McConkey C,Zhao Y,Shetty J,Tran B,Pepper M,Vahedi G,Jenkins MK,McGavern DB,Bosselut R

17-1541-82 was used in Flow Cytometry to identify a transcriptional program that links the CD4+ lineage with Tfh differentiation, a limiting factor for efficient B cell responses.

Tue Sep 17 00:00:00 UTC 2019

CXCR5⁺PD-1⁺ follicular helper CD8 T cells control B cell tolerance.

Nature communications

Chen Y,Yu M,Zheng Y,Fu G,Xin G,Zhu W,Luo L,Burns R,Li QZ,Dent AL,Zhu N,Cui W,Malherbe L,Wen R,Wang D

Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 17-1541-82) in Flow Cytometry

Fri Sep 27 00:00:00 UTC 2019

γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

Nature communications

Rezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HL

17-1541 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

Wed Aug 08 00:00:00 UTC 2018

Helper cell-independent antitumor activity of potent CD8⁺ T cell epitope peptide vaccines is dependent upon CD40L.

Oncoimmunology

Llopiz D,Huarte E,Ruiz M,Bezunartea J,Belsúe V,Zabaleta A,Lasarte JJ,Prieto J,Borrás-Cuesta F,Sarobe P

17-1541-82 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the clinical activity of some potent peptides in peptide vaccines.

Sun Dec 01 00:00:00 UTC 2013

Helper cell-independent antitumor activity of potent CD8⁺ T cell epitope peptide vaccines is dependent upon CD40L.

Oncoimmunology

Llopiz D,Huarte E,Ruiz M,Bezunartea J,Belsúe V,Zabaleta A,Lasarte JJ,Prieto J,Borrás-Cuesta F,Sarobe P

17-1541-82 was used in Flow cytometry/Cell sorting to elucidate the mechanisms underlying the clinical activity of some potent peptides in peptide vaccines.

Sun Dec 01 00:00:00 UTC 2013

The tuberculosis vaccine candidate Bacillus Calmette-Guérin ΔureC::hly coexpressing human interleukin-7 or -18 enhances antigen-specific T cell responses in mice.

PloS one

Rao M,Vogelzang A,Kaiser P,Schuerer S,Kaufmann SH,Gengenbacher M

17-1541 was used in Flow cytometry/Cell sorting to determine whether vaccine strains which express interleukin-7 (hIL)-7 or hIL-18 in the genetic background of BCG δureC::hly modulate specific T cell immunity.

Tue Sep 02 00:00:00 UTC 2014

Surveillance on the status of immune cells after Echinnococcus granulosus protoscoleces infection in Balb/c mice.

PloS one

Pan W,Zhou HJ,Shen YJ,Wang Y,Xu YX,Hu Y,Jiang YY,Yuan ZY,Ugwu CE,Cao JP

17-1541 was used in Flow cytometry/Cell sorting to investigate the systemic immune status of innate and adaptive immunity at 30, 180, 360 days post-infection in mice infected with E. granulousus.

Tue Jan 07 00:00:00 UTC 2014

CD4+ T-cell epitopes associated with antibody responses after intravenously and subcutaneously applied human FVIII in humanized hemophilic E17 HLA-DRB1*1501 mice.

Blood

Steinitz KN,van Helden PM,Binder B,Wraith DC,Unterthurner S,Hermann C,Schuster M,Ahmad RU,Weiller M,Lubich C,de la Rosa M,Schwarz HP,Reipert BM

17-1541 was used in Flow cytometry/Cell sorting to identify FVIII peptides presented by HLA-DRB1*1501.

Thu Apr 26 00:00:00 UTC 2012

DX5+NKT cells display phenotypical and functional differences between spleen and liver as well as NK1.1-Balb/c and NK1.1+ C57Bl/6 mice.

BMC immunology

Werner JM,Busl E,Farkas SA,Schlitt HJ,Geissler EK,Hornung M

17-1541 was used in Flow cytometry/Cell sorting to investigate the expression of several T cell and NK cell markers in spleen and liver DX5+NKT cell subsets in mice.

Fri Apr 29 00:00:00 UTC 2011

Naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival.

Proceedings of the National Academy of Sciences of the United States of America

Lesley R,Kelly LM,Xu Y,Cyster JG

Tue Jul 11 00:00:00 UTC 2006

生物信息学

蛋白别名: CD154; CD40 ligand; CD40-L; H-CD-40-L; M-CD-40-L; RP23-153G22.3; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5

基因别名: CD154; CD40-L; Cd40l; Cd40lg; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; Tnfsf5; TRAP

UniProt ID: (Mouse) P27548

Entrez Gene ID: (Mouse) 21947

功能

cytokine activity tumor necrosis factor receptor binding CD40 receptor binding

参与通路

positive regulation of immune response to tumor cell inflammatory response immune response signal transduction platelet activation B cell differentiation positive regulation of B cell proliferation positive regulation of interferon-gamma production positive regulation of interleukin-10 production positive regulation of interleukin-12 production B cell proliferation negative regulation of apoptotic process isotype switching positive regulation of MHC class II biosynthetic process immunoglobulin secretion regulation of immunoglobulin secretion dendritic cell differentiation positive regulation of endothelial cell apoptotic process

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience™

CD154 (CD40 Ligand) Antibody (17-1541-82) in Flow

产品信息

17-1541-82

免疫组化（冰冻） (IHC (F))

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience™

CD154 (CD40 Ligand) Antibody (17-1541-82) in Flow

产品信息

17-1541-82

免疫组化（冰冻） (IHC (F))

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID