Invitrogen

CD155 Monoclonal Antibody (2H7CD155), PE, eBioscience™

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CD155 Antibody (12-1550-41) in Flow

Staining of U937 cells with Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (blue histogram) or Anti-Human CD155 PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-1550-41

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (2 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

2H7CD155

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11042976

产品详细信息

Description: The monoclonal antibody 2H7CD155 recognizes CD155 also known as the poliovirus receptor. The CD155 protein is a member of the Ig superfamily in which 4 isoforms exist; only the alpha and delta forms are expressed on the membrane of monocytes, prostate cancer cells, as well as neurectodermal and monocytic tumor cells lines. In addition, CD155 is found during mid-gastrulation during embryonic development. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD226 and CD96 are ligands for CD155. Blocking of CD155 is under therapeutic investigation for prevention of variants of poliovirus.

Applications Reported: This 2H7CD155 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2H7CD155 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells or human tumor cell line U937. This can be used at 5 µL (2 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. The external domain mediates cell attachment to the extracellular matrix molecule vitronectin, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. The gene is specific to the primate lineage, and serves as a cellular receptor for poliovirus in the first step of poliovirus replication. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Phenotypic signatures of immune selection in HIV-1 reservoir cells.

Nature

Sun W,Gao C,Hartana CA,Osborn MR,Einkauf KB,Lian X,Bone B,Bonheur N,Chun TW,Rosenberg ES,Walker BD,Yu XG,Lichterfeld M

12-1550-41 was used in Flow cytometry/Cell sorting to phenotypically profile individual HIV-1-infected memory CD4+ T cells from peripheral blood and lymph nodes of people living with HIV-1 and receiving antiretroviral treatment for approximately 10 years.

Wed Feb 01 00:00:00 EST 2023

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

Published figure using CD155 monoclonal antibody (Product # 12-1550-41) in Flow Cytometry

Sat Jan 29 00:00:00 EST 2022

NK cell activation in human hantavirus infection explained by virus-induced IL-15/IL15Rα expression.

PLoS pathogens

Braun M,Björkström NK,Gupta S,Sundström K,Ahlm C,Klingström J,Ljunggren HG

12-1550 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the increased natural killer cell activation and proliferation in Puumala hantavirus infection in humans.