Invitrogen

CD16/CD32 Monoclonal Antibody (93), NovaFluor™ Red 755, eBioscience™

CD16/CD32 Antibody in Flow Cytometry (Flow)

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CD16/CD32 Antibody (M021T03R06-A) in Flow

C57BL/6 mouse bone marrow were unstained (left) or stained with 1 µg of CD16/CD32 Monoclonal Antibody, NovaFluor Red 755 (right). All cells were co-stained with CD45R (B220) Monoclonal Antibody, eFluor 450 (Product # 48-0452-82). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.

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产品信息

M021T03R06-A

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1.0 µg/test

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, lambda

分类

Monoclonal

类型

Antibody

克隆号

偶联物

NovaFluor™ Red 755 NovaFluor™ Red 755 NovaFluor™ Red 755

激发/发射光谱

636/755 nm 查看光谱

形式

Liquid

浓度

0.1 µg/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

RRID

AB_3099017 (AB_2931020)

产品详细信息

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 636 nm; Emission: 756 nm; Laser: 633-640 nm (Red) Laser

靶标信息

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD16A; CD16B; CD32; CD32 receptor 2; Fc gamma receptor IIB; Fc gamma receptor III; Fc gamma RIIB; Fc-gamma RII; fc-gamma RIII; Fc-gamma-RII; fc-gamma-RIIB; Fcg receptor III; FCG2; FcgammaRIIB; FcgammaRIII; FCGR2C; FCGR3A; FCGR3B; FCGRIII; FCR-10; FcRII-b; FcRII-c; fcRIII; FCRIIIA; FGFR2B; IgG Fc receptor II beta; igG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Ly-17; Lymphocyte antigen 17; RP11-5K23.1

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基因别名: AI528646; CD16; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130, (Mouse) 14131

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功能

receptor signaling protein activity protein binding IgG binding immunoglobulin binding IgG receptor activity immunoglobulin receptor superfamily

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