Invitrogen

CD169 (Siglec-1) Monoclonal Antibody (SER-4), APC-eFluor™ 780, eBioscience™

CD169 (Siglec-1) Antibody in Flow Cytometry (Flow)

图: 1 / 3

CD169 (Siglec-1) Antibody (47-5755-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Ly-6G / Ly-6C Monoclonal Antibody, FITC (Product # 11-5931-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or 0.25 µg of CD169 Monoclonal Antibody, APC-eFluor 780 (right). Total cells were used for analysis.

CD169 (Siglec-1) Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

产品信息

47-5755-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Not Applicable

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

SER-4

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2802287

产品详细信息

Description: The SER-4 monoclonal antibody reacts with mouse CD169, which is also known as Sialoadhesin and Siglec-1. CD169 is a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans. This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow. CD169 can also be detected on monocytes in the bone marrow and periphery. This receptor plays a role in cell-cell adhesion, as well as internalization of sialylated virus and bacteria. This endocytic pathway allows CD169+ macrophages to present lipid antigens to iNKT cells, thereby leading to their activation. Studies have also demonstrated that direct interaction of CD169 with regulatory T cells inhibits their proliferation.

The SER-4 antibody has been reported to have antagonist activity.

Applications Reported: This SER-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SER-4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

靶标信息

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

仅用于科研。不用于诊断过程。未经明确授权不得转售。