Invitrogen

CD178 (Fas Ligand) Monoclonal Antibody (MFL3), Biotin, eBioscience™

CD178 (Fas Ligand) Antibody in Flow Cytometry (Flow)

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CD178 (Fas Ligand) Antibody (13-5911-82) in Flow

Staining of mouse Fas Ligand-transfected L5178Y cells with 0.125 µg of Armenian Hamster IgG Isotype Control Biotin (Product # 13-4888-81) (open histogram) or 0.125 µg of Anti-Mouse CD178 (Fas Ligand) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD178 (Fas Ligand) Antibody in Western Blot (WB)

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产品信息

13-5911-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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流式细胞分析 (Flow)

0.25 µg/test

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中和实验 (Neu)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

MFL3

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466794

产品详细信息

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported: The MFL3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 13-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Lymphopenia associated with highly virulent H5N1 virus infection due to plasmacytoid dendritic cell-mediated apoptosis of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Boonnak K,Vogel L,Feldmann F,Feldmann H,Legge KL,Subbarao K

13-5911 was used in Flow cytometry/Cell sorting to examine the mechanism(s) by which lethal H5N1 viruses cause lymphopenia in mammalian hosts.

Sun Jun 15 00:00:00 EDT 2014

Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection.

European journal of immunology

Telieps T,Ewald F,Gereke M,Annemann M,Rauter Y,Schuster M,Ueffing N,von Smolinski D,Gruber AD,Bruder D,Schmitz I

13-5911 was used in Flow cytometry/Cell sorting to study the induction of endogenous murine cellular-FLIPR in activated lymphocytes.

Sat Jun 01 00:00:00 EDT 2013

Kupffer cells potentiate liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1.

Journal of leukocyte biology

Hutchins NA,Wang F,Wang Y,Chung CS,Ayala A

13-5911 was used in Flow cytometry/Cell sorting to study Kupffer cell-mediated potentiation of liver sinusoidal endothelial cell injury in sepsis by ligating programmed cell death ligand-1.

Fri Nov 01 00:00:00 EDT 2013

Induction of rapid apoptosis for class I MHC molecule-restricted CD8(+) HIV-1 gp160-specific murine activated CTLs by free antigenic peptide in vivo.

International immunology

Nakagawa Y,Shimizu M,Norose Y,Takahashi M,Takahashi H

13-5911 was used in Flow cytometry/Cell sorting to provide evidence that exposure to free antigenic peptide leads to the rapid apoptosis of activated virus-specific CD8+ CTLs during infection in vivo.

Tue Jan 01 00:00:00 EST 2013

The magnitude of the T cell response to a clinically significant dose of influenza virus is regulated by TRAIL.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Gurung P,Langlois RA,Hemann EA,Legge KL,Griffith TS

13-5911 was used in Flow cytometry/Cell sorting to study how TRAIL affects the T cell response and ultimately influences the outcome of viral infection.

Tue Nov 01 00:00:00 EDT 2011

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

13-5911 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 EST 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

Thu Jan 01 00:00:00 EST 2009

CD8 T cells utilize TRAIL to control influenza virus infection.

Journal of immunology (Baltimore, Md. : 1950)

Brincks EL,Katewa A,Kucaba TA,Griffith TS,Legge KL

13-5911 was used in Flow cytometry/Cell sorting to determine that TRAIL deficiency decreases CD8(+) T cell-mediated cytotoxicity.

Wed Oct 01 00:00:00 EDT 2008

生物信息学

蛋白别名: Apoptosis (APO 1) antigen ligand 1; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas-LG; generalized lymphoproliferative disease; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

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基因别名: APT1LG1; CD178; CD95-L; CD95L; Fas-L; Fasl; Faslg; gld; Tnfsf6

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UniProt ID: (Mouse) P41047

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Entrez Gene ID: (Mouse) 14103

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功能

death receptor binding cytokine activity tumor necrosis factor receptor binding

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