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CD18 Antibody (MA1805) in Flow
Flow cytometry analysis of CD18 in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA1805) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 4... View More
产品信息
MA1805
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
2E6
抗原
CD18
偶联物
Unconjugated
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_223588
产品详细信息
MA1805 targets CD18 in FACS, IA, and IP applications and shows reactivity with mouse samples.
The MA1805 immunogen is cD18.
MA1805 detects CD18 which has a predicted molecular weight of approximately 83 kDa.
This product is a Low Endotoxin formulation.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
靶标信息
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta
基因别名: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17
UniProt ID: (Mouse) P11835
Entrez Gene ID: (Mouse) 16414
leukocyte migration involved in inflammatory response
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
signal transduction
integrin-mediated signaling pathway
single organismal cell-cell adhesion
natural killer cell activation
neutrophil chemotaxis
heterotypic cell-cell adhesion
receptor clustering
endothelial cell migration
cellular extravasation
positive regulation of nitric oxide biosynthetic process
positive regulation of angiogenesis
regulation of peptidyl-tyrosine phosphorylation
activated T cell proliferation
positive regulation of NF-kappaB transcription factor activity
