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Invitrogen
CD181 (CXCR1) Monoclonal Antibody (eBio8F1-1-4 (8F1-1-4)), eBioscience™
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CD181 (CXCR1) Antibody (14-1819-82) in Flow
Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2b kappa Isotype Control Purified (Product # 14-4732-82) (left) or 0.25 µg of Anti-Human CD181 (CXCR1) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the large scatter population were used for analysis.
产品信息
14-1819-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio8F1-1-4 (8F1-1-4)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_795867
产品详细信息
Description: The eBio8F1-1-4 monoclonal antibody reacts with human CD181 (CXCR1, IL-8Ralpha). CD181 is a 67-70 kDa member of the 7-transmembrane spanning G-protein coupled receptor (GPCR) family. CD181 is expressed as a homodimer, or a heterodimer with CD182 (CXCR2, IL-8Rbeta) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181.
Applications Reported: This eBio8F1-1-4 (8F1-1-4) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio8F1-1-4 (8F1-1-4) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CXCR1 is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 1; CC motif chemokine; CCmotif chemokine; CD181; CDw128a; chemokine (C-X-C motif) receptor 1; CXC; CXC motif chemokine; CXC-R1; CXCR-1; High affinity interleukin-8 receptor A; IL-8 Receptor alpha; IL-8 Receptor IL-RA; IL-8 receptor type 1; IL-8R A; IL-RA; interleukin 8 receptor, alpha; interleukin-8 receptor type 1; interleukin-8 receptor type A
基因别名: C-C; C-C-CKR-1; CD128; CD181; CDw128a; CKR-1; CMKAR1; CXCR1; IL8R1; IL8RA; IL8RBA
UniProt ID: (Human) P25024
Entrez Gene ID: (Human) 3577
