Invitrogen

CD183 (CXCR3) Monoclonal Antibody (CEW33D), PE, eBioscience™

CD183 (CXCR3) Antibody in Flow Cytometry (Flow)

图: 1 / 2

CD183 (CXCR3) Antibody (12-1839-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0038-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD183 (CXCR3) PE (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

产品信息

12-1839-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

CEW33D

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2572589

产品详细信息

Description: The CEW33D monoclonal antibody reacts with human CD183. CD183, also known as CXCR3, is a G protein-coupled chemokine receptor that interacts with ligands CXCL9 (MIG), CXCL10 (IP-10), and CXCL11 (I-TAC). Strongly associated with type 1 immunity, CD183 is induced in naive T cells upon activation and remains upregulated in T helper type (Th)1 cells, CD8 effector cells, NK cells and NKT cells. CD183-ligand interactions mediate infiltration of inflamed tissues in normal type 1 immune responses as well as in many inflammatory and autoimmune diseases. CD183 is also expressed on some B cells and plasmacytoid DC.

Applications Reported: This CEW33D antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CEW33D antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

12-1839 was used in Flow cytometry/Cell sorting to demonstrate that modification of the CK-CKR axis using exogenous recombinant chemokines at the proper time point enhanced CIK cell trafficking ability and improved CIK antitumor effects.

Wed Jul 01 00:00:00 EDT 2020

Chloroquine inhibits human CD4⁺ T-cell activation by AP-1 signaling modulation.

Scientific reports

Schmidt RL,Jutz S,Goldhahn K,Witzeneder N,Gerner MC,Trapin D,Greiner G,Hoermann G,Steiner G,Pickl WF,Burgmann H,Steinberger P,Ratzinger F,Schmetterer KG

12-1839 was used in Flow cytometry/Cell sorting to demonstrate that chloroquine has immunomodulatory effects on CD4(+) T-cells.

Tue Feb 07 00:00:00 EST 2017

Subsets of human CD4(+) regulatory T cells express the peripheral homing receptor CXCR3.

European journal of immunology

Hoerning A,Koss K,Datta D,Boneschansker L,Jones CN,Wong IY,Irimia D,Calzadilla K,Benitez F,Hoyer PF,Harmon WE,Briscoe DM

Published figure using CD183 (CXCR3) monoclonal antibody (Product # 12-1839-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2011

生物信息学

蛋白别名: an; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 3; CC motif chemokine; CCmotif chemokine; CD183; chemokine (C-X-C motif) receptor 3; chemokine receptor 3; CKR-L2; CXC; CXC motif chemokine; CXC-R3; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; IP10; IP10 receptor; Mig receptor

查看更多收起

基因别名: CD182; CD183; CKR-L2; CMKAR3; CXCR3; GPR9; IP10-R; Mig-R; MigR

查看更多收起

UniProt ID: (Human) P49682

查看更多收起

Entrez Gene ID: (Human) 2833

查看更多收起

功能

chemokine receptor activity C-X-C chemokine receptor activity chemokine binding

参与通路