Invitrogen

CD196 (CCR6) Monoclonal Antibody (R6H1), PE, eBioscience™

CD196 (CCR6) Antibody in Flow Cytometry (Flow)

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CD196 (CCR6) Antibody (12-1969-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD196 (CCR6) PE (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

12-1969-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

R6H1

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1272087

产品详细信息

Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R6H1 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

CD39⁺ regulatory T cells accumulate in colon adenocarcinomas and display markers of increased suppressive function.

Oncotarget

Ahlmanner F,Sundström P,Akeus P,Eklöf J,Börjesson L,Gustavsson B,Lindskog EB,Raghavan S,Quiding-Järbrink M

12-1969 was used in Flow cytometry/Cell sorting to treg therapy may hamper development of long-term tolerance and should be approached with caution in the clinical autologous setting.

Tue Dec 11 00:00:00 EST 2018

Selective Expression of CCR10 and CXCR3 by Circulating Human Herpes Simplex Virus-Specific CD8 T Cells.

Journal of virology

Hensel MT,Peng T,Cheng A,De Rosa SC,Wald A,Laing KJ,Jing L,Dong L,Magaret AS,Koelle DM

12-1969 was used in Flow cytometry/Cell sorting to show that CCL27 drives baseline recruitment of HSV-specific CD8 T cells expressing CCR10.

Sun Oct 01 00:00:00 EDT 2017

Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients.

Scientific reports

Ling L,Lin Y,Zheng W,Hong S,Tang X,Zhao P,Li M,Ni J,Li C,Wang L,Jiang Y

12-1969 was used in Flow cytometry/Cell sorting to examine the phenotype, distribution, clinical relevance, and potential function of mucosal associated invariant T cells in colorectal cancer patients.

Wed Feb 03 00:00:00 EST 2016

Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-γ.

Journal of immunology (Baltimore, Md. : 1950)

Sundström P,Ahlmanner F,Akéus P,Sundquist M,Alsén S,Yrlid U,Börjesson L,Sjöling Å,Gustavsson B,Wong SB,Quiding-Järbrink M

12-1969 was used in Flow cytometry/Cell sorting to elucidate the presence and function of mucosa-associated invariant T cells in tumours.

Thu Oct 01 00:00:00 EDT 2015

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

12-1969 was used in Flow cytometry/Cell sorting to investigate how the alteration in chemokine-chemokine receptor expression contributes to pathogen trafficking via myeloid dendritic cells, showing that P.gingivalis infection induces an angiogenic migratory profile and disrupts secondary lymphoid organ-homing CCR7.

Wed Jan 01 00:00:00 EST 2014

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 12-1969-42) in Flow Cytometry

Fri Oct 13 00:00:00 EDT 2017

生物信息学

蛋白别名: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22

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基因别名: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22

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UniProt ID: (Human) P51684

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Entrez Gene ID: (Human) 1235

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功能

receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity C-C chemokine binding

参与通路