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Invitrogen
CD196 (CCR6) Monoclonal Antibody (R6H1), Brilliant Violet™ 421, eBioscience™
图: 1 / 1
CD196 (CCR6) Antibody (404-1969-42) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, PE (Product # 12-0199-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 421 (Product # 404-4714-81) (left) or CD196 (CCR6) Monoclonal Antibody, Brilliant Violet 421 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
404-1969-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
R6H1
偶联物
激发/发射光谱
406/423 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.
Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This R6H1 antibody has been pre-diluted and tested by flow cytometric analysis of . This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.™
靶标信息
CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22
基因别名: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22
UniProt ID: (Human) P51684
Entrez Gene ID: (Human) 1235
dendritic cell chemotaxis
leukocyte migration involved in inflammatory response
movement of cell or subcellular component
chemotaxis
immune response
humoral immune response
cellular defense response
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of epithelial cell migration
calcium-mediated signaling
isotype switching to IgA isotypes
cell chemotaxis
positive regulation of sperm motility involved in capacitation
chemokine-mediated signaling pathway
lymphocyte migration
T cell migration
thymocyte migration
DN2 thymocyte differentiation
DN3 thymocyte differentiation
regulation of T cell migration
positive regulation of dendritic cell chemotaxis
