Invitrogen

CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), PE, eBioscience™

CD201 (EPCR) Antibody in Flow Cytometry (Flow)

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CD201 (EPCR) Antibody (12-2012-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Ly-6A/E (Sca-1) Monoclonal Antibody, APC (Product # 17-5981-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, PE (Product # 12-4031-83) (left) or 0.25 µg of CD201 Monoclonal Antibody, PE (right). Cells were also stained with Anti-Mouse CD117 (c-Kit) PE-Cy7 (Product # 25-1171-82). Lineage negative/low, CD117+ cells in the large scatter population were used for analysis.

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产品信息

12-2012-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看16篇已发表文章 16篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio1560 (1560)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_914317

产品详细信息

Description: The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.

Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.

Applications Reported: This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio1560 (1560) antibody has been tested by flow cytometric analysis of the bEnd.3 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Macrophages maintain mammary stem cell activity and mammary homeostasis via TNF-α-PI3K-Cdk1/Cyclin B1 axis.

NPJ Regenerative medicine

Zhou Y,Ye Z,Wei W,Zhang M,Huang F,Li J,Cai C

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Tue May 02 00:00:00 EDT 2023

PU.1 Expression Defines Distinct Functional Activities in the Phenotypic HSC Compartment of a Murine Inflammatory Stress Model.

Cells

Chavez JS,Rabe JL,Hernandez G,Mills TS,Niño KE,Davizon-Castillo P,Pietras EM

12-2012-82 was used in Flow Cytometry to show PU.1 levels are linked to hematopoietic stem cells fate in steady state and inflammatory stress conditions.

Tue Feb 15 00:00:00 EST 2022

Programmed Cell Death Ligand 1 Is Enriched in Mammary Stem Cells and Promotes Mammary Development and Regeneration.

Frontiers in cell and developmental biology

Wang R,Huang F,Wei W,Zhou Y,Ye Z,Yu L,Hu J,Cai C

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Lentiviral CRISPR-guided RNA library screening identified Adam17 as an upstream negative regulator of Procr in mammary epithelium.

BMC biotechnology

Wu T,Wang Y,Xiao T,Ai Y,Li J,Zeng YA,Yu QC

12-2012-82 was used in Flow cytometry/Cell sorting to establish an efficient CRISPR-Cas9/FACS screening system, which identifies the regulators of membrane proteins.

Mon Jul 19 00:00:00 EDT 2021

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr⁺ Progenitors.

Cell

Wang D,Wang J,Bai L,Pan H,Feng H,Clevers H,Zeng YA

12-2012-82 was used in Flow Cytometry to demonstrate that the adult mouse pancreatic islet contains a population of Procr+ endocrine progenitors.

Thu Mar 19 00:00:00 EDT 2020

Protein C receptor is a therapeutic stem cell target in a distinct group of breast cancers.

Cell research

Wang D,Hu X,Liu C,Jia Y,Bai Y,Cai C,Wang J,Bai L,Yang R,Lin C,Liu YR,Li S,Qiao F,Yao L,Chen L,Ge G,Jiang H,Li D,Li L,Chen J,Shao ZM,Zeng YA

12-2012 was used in Flow cytometry/Cell sorting to suggest a key role of MaSC in breast tumorigenesis.

Tue Oct 01 00:00:00 EDT 2019

Immune synapses between mast cells and γδ T cells limit viral infection.

The Journal of clinical investigation

Mantri CK,St John AL

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Procr-expressing progenitor cells are responsible for murine ovulatory rupture repair of ovarian surface epithelium.

Nature communications

Wang J,Wang D,Chu K,Li W,Zeng YA

12-2012 was used in Flow cytometry/Cell sorting to show that the expression of Protein C Receptor (Procr) marks a progenitor population in adult mice that is responsible for OSE repair post ovulation.

Thu Oct 31 00:00:00 EDT 2019

Adhesion Assay for Murine Bone Marrow Hematopoietic Stem Cells.

Bio-protocol

Avci S,Gur-Cohen S,Avemaria F,Lapidot T

12-2012-82 was used in Flow Cytometry to introduce an adhesion assay optimized for VLA4 expressing murine bone marrow stem cells.

Mon Feb 20 00:00:00 EST 2017

Mll-AF4 Confers Enhanced Self-Renewal and Lymphoid Potential during a Restricted Window in Development.

Cell reports

Barrett NA,Malouf C,Kapeni C,Bacon WA,Giotopoulos G,Jacobsen SEW,Huntly BJ,Ottersbach K

12-2012 was used in Flow cytometry/Cell sorting to elucidate the contribution of MII-AF4 in B cell acute lymphoblastic leukaemia early development in the mouse embryo.

Tue Jul 26 00:00:00 EDT 2016

PAR1 signaling regulates the retention and recruitment of EPCR-expressing bone marrow hematopoietic stem cells.

Nature medicine

Gur-Cohen S,Itkin T,Chakrabarty S,Graf C,Kollet O,Ludin A,Golan K,Kalinkovich A,Ledergor G,Wong E,Niemeyer E,Porat Z,Erez A,Sagi I,Esmon CT,Ruf W,Lapidot T

12-2012 was used in Flow cytometry/Cell sorting to study long-term repopulating hematopoietic stem cells, showing that PAR1 signaling regulates the retention and recruitment of EPCR-expressing hematopoietic stem cells.

Sun Nov 01 00:00:00 EDT 2015

Acute DNA damage activates the tumour suppressor p53 to promote radiation-induced lymphoma.

Nature communications

Lee CL,Castle KD,Moding EJ,Blum JM,Williams N,Luo L,Ma Y,Borst LB,Kim Y,Kirsch DG

12-2012 was used in Flow cytometry/Cell sorting to show that total-body irradiation promotes the expansion of a rare population of thymocytes that express oncogenic Kras(G12D).

Thu Sep 24 00:00:00 EDT 2015

Oral or parenteral administration of curcumin does not prevent the growth of high-risk t(4;11) acute lymphoblastic leukemia cells engrafted into a NOD/SCID mouse model.

International journal of oncology

Zunino SJ,Storms DH,Newman JW,Pedersen TL,Keen CL,Ducore JM

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Fri Feb 01 00:00:00 EST 2013

Pediatric glioma stem cells: biologic strategies for oncolytic HSV virotherapy.

Frontiers in oncology

Friedman GK,Raborn J,Kelly VM,Cassady KA,Markert JM,Gillespie GY

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Mon Mar 04 00:00:00 EST 2013

6-Formylindolo (3,2-b)carbazole (FICZ) enhances retinoic acid (RA)-induced differentiation of HL-60 myeloblastic leukemia cells.

Molecular cancer

Bunaciu RP,Yen A

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Thu May 09 00:00:00 EDT 2013

Dual functions of the C5a receptor as a connector for the K562 erythroblast-like cell-THP-1 macrophage-like cell island and as a sensor for the differentiation of the K562 erythroblast-like cell during haemin-induced erythropoiesis.

Clinical & developmental immunology

Nishiura H,Zhao R,Yamamoto T

Published figure using CD201 (EPCR) monoclonal antibody (Product # 12-2012-82) in Flow Cytometry

Mon Aug 12 00:00:00 EDT 2013

生物信息学

蛋白别名: Activated protein C receptor; APC receptor; bA42O4.2; CD antigen CD201; CD201; Centrocyclin; Centrosomal protein CCD41; Endothelial cell protein C receptor; Endothelial protein C receptor

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基因别名: AI325044; Ccca; Ccd41; Epcr; Procr

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UniProt ID: (Mouse) Q64695

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Entrez Gene ID: (Mouse) 19124

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功能

protein C-terminus binding protein-binding activity modulator

参与通路

immune response blood coagulation hemostasis antigen processing and presentation negative regulation of coagulation