Invitrogen

CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), APC, eBioscience™

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产品信息

17-2099-42

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test
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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rat / IgG2a, kappa

推荐的同型对照

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

eB-h209

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱 spectra

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11039758

产品详细信息

Description: The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.

Applications Reported: The eB-h209 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; CD209; CD209 antigen; DC-SIGN; Dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin; HIV gpl20-binding protein; MGC129965; MGC130443

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基因别名: CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1

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UniProt ID: (Human) Q9NNX6

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Entrez Gene ID: (Human) 30835

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