Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD209b (SIGN-R1) Monoclonal Antibody (eBio22D1 (22D1)), Functional Grade, eBioscience™
图: 1 / 6
CD209b (SIGN-R1) Antibody (16-2093-82) in Flow
Staining of C57Bl/6 splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.5 µg of Anti-Mouse CD209b (SIGN-R1) Functional Grade Purified (filled histogram) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83).Cells in the lymphocyte and monocyte gates were used for analysis, and events displayed are gated on CD11c+ cells.
产品信息
16-2093-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
eBio22D1 (22D1)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_795855
产品详细信息
Description: The eBio22D1 monoclonal antibody reacts with mouse SIGNR1 (CD209b). SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase. The eBio22D1 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.
Applications Reported: This eBio22D1 (22D1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and ELISA. in vivo injection of 22D1 has been shown to induce temporary knockdown of SIGNR1 expression.
Applications Tested: This eBio22D1 (22D1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD209; CD209 antigen-like protein A; CD209 antigen-like protein B; DC SIGNR1; DC-SIGN; DC-SIGN-related protein 1; DC-SIGNR1; Dendritic cell-specific ICAM-3-grabbing non-integrin; MGC130443; OtB7
基因别名: 1810030I22Rik; CD209; Cd209a; Cd209b; CDSIGN; CIRE; DC-SIGN; DC-SIGN1; DC-SIGNR1; Dcsign; mSIGNR1; OtB7; SIGN-R1; SIGNR1; SIGNR5
UniProt ID: (Mouse) Q91ZX1, (Mouse) Q8CJ91
Entrez Gene ID: (Mouse) 170786, (Mouse) 69165
endocytosis
regulation of gene expression
regulation of blood coagulation
regulation of T cell proliferation
viral entry into host cell
detection of yeast
phagocytosis, recognition
detection of bacterium
positive regulation of tumor necrosis factor biosynthetic process
positive regulation of phagocytosis
