Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD217 (IL-17Ra) Monoclonal Antibody (J10MBS), PE, eBioscience™
图: 1 / 2
CD217 (IL-17Ra) Antibody (12-7517-42) in Flow
Human peripheral blood was lysed with 1X RBC Lysis Buffer (00-4333) and stained with Anti-Human CD4 FITC (Product # 11-0049-42), Anti-Human CD10 APC (Product # 17-0106-42) and Anti-Human IL-17 Receptor PE. CD4 (hi) T cells (blue), CD4 (intermediate) monocytes (purple), and CD10+ granulocytes (orange) were gated (left) and analyzed for IL-17 Receptor staining (right).
产品信息
12-7517-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
J10MBS
偶联物
激发/发射光谱
565/576 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with 0.2% BSA
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2043872
产品详细信息
Description: The J10MBS monoclonal antibody reacts with human interleukin 17 receptor A (IL-17RA, IL-17R). IL-17RA is a type I membrane protein of approximately 96 kDa (866 amino acids) and is involved in inflammatory responses to IL-17A, IL-17F and IL-17AF heterodimer. It forms heterodimeric complexes with several other members of its receptor family, including IL-17RC for IL-17A and IL-17F signaling and IL-17RC for IL-17E (IL-25) signaling. IL-17RA is ubiquitously expressed, but in leukocytes its expression is elevated on granulocytes and monocytes.
Applications Reported: This J10MBS antibody has been reported for use in flow cytometric analysis.
Applications Tested: This J10MBS antibody has been pre-titrated and tested by flow cytometric analysis of human lysed whole blood. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD217; CDw217; IL-17 receptor A; Interleukin-17 receptor A; MGC10262
基因别名: CANDF5; CD217; CDw217; hIL-17R; IL-17RA; IL17R; IL17RA
UniProt ID: (Human) Q96F46
Entrez Gene ID: (Human) 23765
cell surface receptor signaling pathway
cytokine-mediated signaling pathway
positive regulation of interleukin-23 production
defense response to fungus
granulocyte chemotaxis
fibroblast activation
positive regulation of cytokine production involved in inflammatory response
positive regulation of interleukin-5 secretion
positive regulation of interleukin-13 secretion
