Invitrogen

CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE-Cyanine7, eBioscience™

CD223 (LAG-3) Antibody in Flow Cytometry (Flow)

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CD223 (LAG-3) Antibody (25-2231-82) in Flow

Staining of 3-day Anti-Mouse CD3e and CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.5 µg of Rat IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4301-82) (blue histogram) or 0.5 µg of Anti-Mouse CD223 (Lag-3) PE-Cyanine7 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

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产品信息

25-2231-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioC9B7W (C9B7W)

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573428

产品详细信息

Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.

CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.

Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

BRCA1 deficiency in mature CD8⁺ T lymphocytes impairs antitumor immunity.

Journal for immunotherapy of cancer

Wu B,Qi L,Chiang HC,Pan H,Zhang X,Greenbaum A,Stark E,Wang LJ,Chen Y,Haddad BR,Clagett D,Isaacs C,Elledge R,Horvath A,Hu Y,Li R

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

BRCA1 deficiency in mature CD8⁺ T lymphocytes impairs antitumor immunity.

Journal for immunotherapy of cancer

Wu B,Qi L,Chiang HC,Pan H,Zhang X,Greenbaum A,Stark E,Wang LJ,Chen Y,Haddad BR,Clagett D,Isaacs C,Elledge R,Horvath A,Hu Y,Li R

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Therapeutic KRAS^G12C inhibition drives effective interferon-mediated antitumor immunity in immunogenic lung cancers.

Science advances

Mugarza E,van Maldegem F,Boumelha J,Moore C,Rana S,Llorian Sopena M,East P,Ambler R,Anastasiou P,Romero-Clavijo P,Valand K,Cole M,Molina-Arcas M,Downward J

25-2231-82 was used in Flow cytometry/Cell sorting to characterized how KRASG12C inhibition reverses immunosuppression driven by oncogenic KRAS in a number of preclinical lung cancer models with varying levels of immunogenicity.

Fri Jul 22 00:00:00 EDT 2022

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Cell

Gangoso E,Southgate B,Bradley L,Rus S,Galvez-Cancino F,McGivern N,Güç E,Kapourani CA,Byron A,Ferguson KM,Alfazema N,Morrison G,Grant V,Blin C,Sou I,Marques-Torrejon MA,Conde L,Parrinello S,Herrero J,Beck S,Brandner S,Brennan PM,Bertone P,Pollard JW,Quezada SA,Sproul D,Frame MC,Serrels A,Pollard SM

25-2231-82 was used in Flow Cytometry to conclude that epigenetic immunoediting may drive an acquired immune evasion program in the most aggressive mesenchymal GBM subtype by reshaping the tumor immune microenvironment.

Thu Apr 29 00:00:00 EDT 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Human prostate cancer bone metastases have an actionable immunosuppressive microenvironment.

Cancer cell

Kfoury Y,Baryawno N,Severe N,Mei S,Gustafsson K,Hirz T,Brouse T,Scadden EW,Igolkina AA,Kokkaliaris K,Choi BD,Barkas N,Randolph MA,Shin JH,Saylor PJ,Scadden DT,Sykes DB,Kharchenko PV

25-2231-82 was used in Flow cytometry/Cell sorting to define distinct features of the bone marrow (BM) microenvironment by analyzing single cells from bone metastatic prostate tumors, involved BM, uninvolved BM, and BM from cancer-free, orthopedic patients, and healthy individuals.

Mon Nov 08 00:00:00 EST 2021

Role of Regulatory T Cell and Effector T Cell Exhaustion in Liver-Mediated Transgene Tolerance in Muscle.

Molecular therapy. Methods & clinical development

Poupiot J,Costa Verdera H,Hardet R,Colella P,Collaud F,Bartolo L,Davoust J,Sanatine P,Mingozzi F,Richard I,Ronzitti G

25-2231 was used in Flow cytometry/Cell sorting to characterise liver-induced transgene tolerance after simultaneous transduction of liver and muscle.

Fri Dec 13 00:00:00 EST 2019

LAG-3 Inhibitory Receptor Expression Identifies Immunosuppressive Natural Regulatory Plasma Cells.

Immunity

Lino AC,Dang VD,Lampropoulou V,Welle A,Joedicke J,Pohar J,Simon Q,Thalmensi J,Baures A,Flühler V,Sakwa I,Stervbo U,Ries S,Jouneau L,Boudinot P,Tsubata T,Adachi T,Hutloff A,Dörner T,Zimber-Strobl U,de Vos AF,Dahlke K,Loh G,Korniotis S,Goosmann C,Weill JC,Reynaud CA,Kaufmann SHE,Walter J,Fillatreau S

25-2231 was used in Flow cytometry/Cell sorting to identify a natural plasma cell subset that distinctively expresses the inhibitory receptor LAG-3 and mediates this function in vivo.

Tue Jul 17 00:00:00 EDT 2018

Rational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.

Nature communications

Liu Z,Ravindranathan R,Kalinski P,Guo ZS,Bartlett DL

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Mon Mar 27 00:00:00 EDT 2017

Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments.

Cell

Di Marco Barros R,Roberts NA,Dart RJ,Vantourout P,Jandke A,Nussbaumer O,Deban L,Cipolat S,Hart R,Iannitto ML,Laing A,Spencer-Dene B,East P,Gibbons D,Irving PM,Pereira P,Steinhoff U,Hayday A

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using CD223 (LAG-3) monoclonal antibody (Product # 25-2231-82) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

生物信息学

蛋白别名: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; LAG-3; Lymphocyte activation gene 3 protein; sLAG 3; sLAG3; soluble LAG 3lymphocyte activating 3; soluble LAG3

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基因别名: CD223; LAG-3; Lag3; Ly66

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UniProt ID: (Mouse) Q61790

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Entrez Gene ID: (Mouse) 16768

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功能

transmembrane signaling receptor activity MHC class II protein binding transmembrane signal receptor

参与通路

cell surface receptor signaling pathway negative regulation of interleukin-2 biosynthetic process positive regulation of natural killer cell mediated cytotoxicity negative regulation of T cell activation