Invitrogen

CD243 (ABCB1) Monoclonal Antibody (UIC2), PE, eBioscience™

CD243 (ABCB1) Antibody in Flow Cytometry (Flow)

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CD243 (ABCB1) Antibody (12-2439-42) in Flow

Staining of the 8226/S (left) and 8226 DOX40 (right) cell lines with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human CD243 (ABCB1) PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-2439-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human

已发表种属

Dog, Human

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

UIC2

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1603172

产品详细信息

Description: This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes.

This antibody has been reported to inhibit MDR1-mediated efflux.

Applications Reported: This UIC2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UIC2 antibody has been pre-titrated and tested by flow cytometric analysis on multidrug resistant cell lines. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype.

Stem cells international

Boesch M,Wolf D,Sopper S

Published figure using CD243 (ABCB1) monoclonal antibody (Product # 12-2439-42) in Flow Cytometry

Fri Jan 22 00:00:00 EST 2016

Canine osteosarcoma cells exhibit resistance to aurora kinase inhibitors.

Veterinary and comparative oncology

Cannon CM,Pozniak J,Scott MC,Ito D,Gorden BH,Graef AJ,Modiano JF

12-2439 was used in Flow cytometry/Cell sorting to investigate the effect of Aurora kinase inhibitors AZD1152 and VX680 on canine osteosarcoma cells.

Sun Mar 01 00:00:00 EST 2015

Translational phase I trial of vorinostat (suberoylanilide hydroxamic acid) combined with cytarabine and etoposide in patients with relapsed, refractory, or high-risk acute myeloid leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research

Gojo I,Tan M,Fang HB,Sadowska M,Lapidus R,Baer MR,Carrier F,Beumer JH,Anyang BN,Srivastava RK,Espinoza-Delgado I,Ross DD

12-2439 was used in Flow cytometry/Cell sorting to determine the maximum-tolerated dose of the histone deacetylase inhibitor vorinostat combined with fixed doses of cytarabine and etoposide in patients with poor-risk or advanced acute leukemia.

Mon Apr 01 00:00:00 EDT 2013

Ethyl 2-amino-6-(3,5-dimethoxyphenyl)-4-(2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (CXL017): a novel scaffold that resensitizes multidrug resistant leukemia cells to chemotherapy.

ACS chemical biology

Das SG,Hermanson DL,Bleeker N,Lowman X,Li Y,Kelekar A,Xing C

12-2439 was used in Flow cytometry/Cell sorting to present new evidence for treatment of MDR cancers and offer mechanistic insights toward its preferential targeting of drug-resistant cancer.

Fri Feb 15 00:00:00 EST 2013

Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

PloS one

Rosu-Myles M,She YM,Fair J,Muradia G,Mehic J,Menendez P,Prasad SS,Cyr TD

12-2439 was used in Flow cytometry/Cell sorting to provide evidence of heterogeneity among bone marrow stromal cells and identify potential candidate proteins for quantifying MSCs in vitro.

Thu Dec 13 00:00:00 EST 2012

生物信息学

蛋白别名: ATP-binding cassette sub-family B member 1; ATP-binding cassette, sub-family B (MDR/TAP), member 1; ATP-dependent translocase ABCB1; CD243; colchicin sensitivity; doxorubicin resistance; Multidrug resistance protein 1; P-glycoprotein 1; Phospholipid transporter ABCB1

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基因别名: ABC20; ABCB1; CD243; CLCS; GP170; MDR1; P-GP; PGY1

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UniProt ID: (Human) P08183

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Entrez Gene ID: (Human) 5243

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功能

transporter activity protein binding ATP binding xenobiotic-transporting ATPase activity ATPase activity, coupled to transmembrane movement of substances ATP-binding cassette (ABC) transporter

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