Invitrogen

CD253 (TRAIL) Monoclonal Antibody (N2B2), PE, eBioscience™

CD253 (TRAIL) Antibody in Flow Cytometry (Flow)

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CD253 (TRAIL) Antibody (12-5951-82) in Flow

Staining of mouse CD253 transfected cells with 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (blue histogram) or 0.06 µg of Anti-Mouse CD253 (TRAIL) PE (purple histogram). Total viable cells were used for analysis.

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产品信息

12-5951-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

N2B2

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466057

产品详细信息

Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

Applications Reported: N2B2 has been reported for use in flow cytometric analysis.

Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse CD253 transfected cells and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells.

Cell reports

Dodard G,Tata A,Erick TK,Jaime D,Miah SMS,Quatrini L,Escalière B,Ugolini S,Vivier E,Brossay L

12-5951 was used in Flow cytometry/Cell sorting to characterize the kinetics and immune response of each of the hepatic NK cell populations to viral challenge and other stimuli.

Tue Jul 07 00:00:00 EDT 2020

Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression.

Cell metabolism

Cong J,Wang X,Zheng X,Wang D,Fu B,Sun R,Tian Z,Wei H

12-5951 was used in Flow cytometry/Cell sorting to identify novel mechanisms in natural killer cell dysfunction.

Tue Aug 07 00:00:00 EDT 2018

Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation.

PloS one

Dang VT,Tanabe K,Tanaka Y,Tokumoto N,Misumi T,Saeki Y,Fujikuni N,Ohdan H

12-5951 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms underlying the enhancement of natural killer cell function by fasting in mice.

Mon Jun 29 00:00:00 EDT 2015

Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells.

PloS one

Ikeda T,Hirata S,Takamatsu K,Haruta M,Tsukamoto H,Ito T,Uchino M,Ando Y,Nagafuchi S,Nishimura Y,Senju S

12-5951 was used in Flow cytometry/Cell sorting to investigate the effect of embryonic stem cell-derived dendritic cells on autoimmunity, showing that suppression of Th1-mediated autoimmunity occurs in NOD and EAE mice.

Thu Dec 17 00:00:00 EST 2015

Enhanced effector responses in activated CD8+ T cells deficient in diacylglycerol kinases.

Cancer research

Riese MJ,Wang LC,Moon EK,Joshi RP,Ranganathan A,June CH,Koretzky GA,Albelda SM

12-5951 was used in Flow cytometry/Cell sorting to investigate whether activation of T cells using chimeric antigen receptor can be enhanced by deletion of negative regulators, showing that deletion of dgk enhanced cytokine production and were effective at limiting growth of implanted tumours.

Sat Jun 15 00:00:00 EDT 2013

TRAIL/DR5 plays a critical role in NK cell-mediated negative regulation of dendritic cell cross-priming of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Iyori M,Zhang T,Pantel H,Gagne BA,Sentman CL

12-5951 was used in Flow cytometry/Cell sorting to indicate a novel alternative pathway for DC/NK cell interactions in antitumor immunity.

Thu Sep 15 00:00:00 EDT 2011

Peptide vaccine induces enhanced tumor growth associated with apoptosis induction in CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Muraoka D,Kato T,Wang L,Maeda Y,Noguchi T,Harada N,Takeda K,Yagita H,Guillaume P,Luescher I,Old LJ,Shiku H,Nishikawa H

12-5951 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying the failure of anti-tumour vaccination using synthetic peptides, showing that it induces enhanced tumour growth associated with apoptosis induction in CD8+ T cells.

Wed Sep 15 00:00:00 EDT 2010

Increased killing of liver NK cells by Fas/Fas ligand and NKG2D/NKG2D ligand contributes to hepatocyte necrosis in virus-induced liver failure.

Journal of immunology (Baltimore, Md. : 1950)

Zou Y,Chen T,Han M,Wang H,Yan W,Song G,Wu Z,Wang X,Zhu C,Luo X,Ning Q

12-5951 was used in Flow cytometry/Cell sorting to reveal a pivotal role for liver natural killer cells in the pathogenesis of virus-induced fulminant hepatic failure and acute-on-chronic liver failure.

Fri Jan 01 00:00:00 EST 2010

Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS

12-5951 was used in Flow cytometry/Cell sorting to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs.

Thu Jan 01 00:00:00 EST 2009

Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease.

Proceedings of the National Academy of Sciences of the United States of America

Takeda K,Kojima Y,Ikejima K,Harada K,Yamashina S,Okumura K,Aoyama T,Frese S,Ikeda H,Haynes NM,Cretney E,Yagita H,Sueyoshi N,Sato N,Nakanuma Y,Smyth MJ,Okumura K

12-5951 was used in Flow cytometry/Cell sorting to show that the death signal mediated by TRAIL receptor 2/death receptor 5 may regulate of cholestatic liver injury.

Tue Aug 05 00:00:00 EDT 2008

Type I IFN-induced, NKT cell-mediated negative control of CD8 T cell priming by dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Bochtler P,Kröger A,Schirmbeck R,Reimann J

12-5951-82 was used in Flow cytometry/Cell sorting to investigated the negative effect of type I IFN (IFN-I) on the priming of specific CD8 T cell immunity.

Fri Aug 01 00:00:00 EDT 2008

生物信息学

蛋白别名: CD253; CD253 antigen; Protein TRAIL; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL protein; TRAIL/APO2L; Tumor necrosis factor ligand superfamily member 10

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基因别名: A330042I21Rik; AI448571; APO-2L; Ly81; TL2; Tnfsf10; Trail

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UniProt ID: (Mouse) P50592

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Entrez Gene ID: (Mouse) 22035

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功能

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

参与通路

apoptotic process immune response positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of release of cytochrome c from mitochondria positive regulation of extrinsic apoptotic signaling pathway