Invitrogen

CD253 (TRAIL) Monoclonal Antibody (N2B2), Functional Grade, eBioscience™

CD253 (TRAIL) Antibody in Flow Cytometry (Flow)

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CD253 (TRAIL) Antibody (16-5951-85) in Flow

Staining of mouse TRAIL transfected cells with Anti-Mouse CD253 (TRAIL) PE. Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

CD253 (TRAIL) Antibody in Neutralization (Neu)

CD253 (TRAIL) Antibody in Inhibition Assays (IA)

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产品信息

16-5951-85

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看7篇已发表文章 7篇已发表文章

中和实验 (Neu)

Assay-Dependent

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功能检测 (Functional)

Assay-Dependent

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抑制试剂 (IA)

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阻断反应 (BLOCK)

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

N2B2

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469165

产品详细信息

Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

Applications Reported: N2B2 has been reported for use in flow cytometric analysis, and blocking of TRAIL in functional assays.

Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CD8⁺ T cells maintain killing of MHC-I-negative tumor cells through the NKG2D-NKG2DL axis.

Nature cancer

Lerner EC,Woroniecka KI,D'Anniballe VM,Wilkinson DS,Mohan AA,Lorrey SJ,Waibl-Polania J,Wachsmuth LP,Miggelbrink AM,Jackson JD,Cui X,Raj JA,Tomaszewski WH,Cook SL,Sampson JH,Patel AP,Khasraw M,Gunn MD,Fecci PE

16-5951-85 was used in Blocking Assay to report that CD8+ T cells maintain the capacity to kill tumor cells that are entirely devoid of MHC-I expression.

Fri Sep 01 00:00:00 EDT 2023

CD8⁺ T cells maintain killing of MHC-I-negative tumor cells through the NKG2D-NKG2DL axis.

Nature cancer

16-5951-85 was used in Blocking Assay to report that CD8+ T cells maintain the capacity to kill tumor cells that are entirely devoid of MHC-I expression.

Fri Sep 01 00:00:00 EDT 2023

Gut-licensed IFNγ⁺ NK cells drive LAMP1⁺TRAIL⁺ anti-inflammatory astrocytes.

Nature

Sanmarco LM,Wheeler MA,Gutiérrez-Vázquez C,Polonio CM,Linnerbauer M,Pinho-Ribeiro FA,Li Z,Giovannoni F,Batterman KV,Scalisi G,Zandee SEJ,Heck ES,Alsuwailm M,Rosene DL,Becher B,Chiu IM,Prat A,Quintana FJ

16-5951-85 was used in Flow Cytometry to show that LAMP1+TRAIL+ astrocytes limit CNS inflammation by inducing T cell apoptosis, and that this astrocyte subset is maintained by meningeal IFNγ+ NK cells that are licensed by the microbiome.

Mon Feb 01 00:00:00 EST 2021

Dependence of innate lymphoid cell 1 development on NKp46.

PLoS biology

Wang Y,Dong W,Zhang Y,Caligiuri MA,Yu J

Published figure using CD253 (TRAIL) monoclonal antibody (Product # 16-5951-85) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Rapid loss of group 1 innate lymphoid cells during blood stage Plasmodium infection.

Clinical & translational immunology

Ng SS,Souza-Fonseca-Guimaraes F,Rivera FL,Amante FH,Kumar R,Gao Y,Sheel M,Beattie L,Montes de Oca M,Guillerey C,Edwards CL,Faleiro RJ,Frame T,Bunn PT,Vivier E,Godfrey DI,Pellicci DG,Lopez JA,Andrews KT,Huntington ND,Smyth MJ,McCarthy J,Engwerda CR

16-5951 was used in Flow cytometry/Cell sorting to characterise the role of group 1 ILCs during Plasmodium infection.

Wed Sep 30 00:00:00 EDT 2020

Deficiency in the mitochondrial apoptotic pathway reveals the toxic potential of autophagy under ER stress conditions.

Autophagy

Deegan S,Saveljeva S,Logue SE,Pakos-Zebrucka K,Gupta S,Vandenabeele P,Bertrand MJ,Samali A

16-5951-85 was used in Inhibition experiments to study the toxic potential of autophagy under endoplasmic reticulum stress conditions.

Mon Sep 14 00:00:00 EDT 2015

RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions.

Cell death & disease

Estornes Y,Aguileta MA,Dubuisson C,De Keyser J,Goossens V,Kersse K,Samali A,Vandenabeele P,Bertrand MJ

Published figure using CD253 (TRAIL) monoclonal antibody (Product # 16-5951-85) in Neutralization

Thu Dec 04 00:00:00 EST 2014

Modulation of natural killer cell antitumor activity by the aryl hydrocarbon receptor.

Proceedings of the National Academy of Sciences of the United States of America

Shin JH,Zhang L,Murillo-Sauca O,Kim J,Kohrt HE,Bui JD,Sunwoo JB

16-5951 was used in Flow cytometry/Cell sorting to explore the regulation of natural killer cells by the aryl hydrocarbon receptor (AhR).

Tue Jul 23 00:00:00 EDT 2013

Immune unresponsiveness to secondary heterologous bacterial infection after sepsis induction is TRAIL dependent.

Journal of immunology (Baltimore, Md. : 1950)

Gurung P,Rai D,Condotta SA,Babcock JC,Badovinac VP,Griffith TS

16-5951 was used in Blocking experiments to implicate TRAIL-dependent immune suppression during sepsis, suggesting TRAIL neutralization may be a potential therapeutic target to restore cellular immunity.

Thu Sep 01 00:00:00 EDT 2011

Latent herpesvirus infection arms NK cells.

Blood

White DW,Keppel CR,Schneider SE,Reese TA,Coder J,Payton JE,Ley TJ,Virgin HW,Fehniger TA

16-5951 was used in Flow cytometry/Cell sorting to investigate the immune environment created by latent herpesvirus infection, and how this may provide a mechanism whereby host NK-cell function is enhanced in vivo..

Thu Jun 03 00:00:00 EDT 2010

Migratory and antigen presentation functions of IFN-producing killer dendritic cells.

Cancer research

Himoudi N,Yan M,Bouma G,Morgenstern D,Wallace R,Seddon B,Buddle J,Eddaoudi A,Howe SJ,Cooper N,Anderson J

16-5951 was used in Flow cytometry/Cell sorting to study how BM-IKDCs are capable of cytotoxic killing of tumour targets, and also of potent antigen presentation after encountering antigen in the context of a viable target cell.

Sat Aug 15 00:00:00 EDT 2009

Interleukin-15 liver gene transfer increases the number and function of IKDCs and NK cells.

Gene therapy

Arina A,Murillo O,Dubrot J,Azpilikueta A,Gabari I,Perez-Gracia JL,Alfaro C,Berasain C,Prieto J,Ferrini S,Hervas-Stubbs S,Melero I

16-5951 was used in Flow cytometry/Cell sorting to show the novel functional link between circulating IL-15 and interferon (IFN)-producing killer dendritic cells (IKDCs).

Tue Apr 01 00:00:00 EDT 2008

Tumour necrosis factor related apoptosis inducing ligand (TRAIL) induces hepatic steatosis in viral hepatitis and after alcohol intake.

Gut

Mundt B,Wirth T,Zender L,Waltemathe M,Trautwein C,Manns MP,Kühnel F,Kubicka S

16-5951 was used in Functional assays to investigate the role of tumour necrosis factor related apoptosis inducing ligand in viral hepatitis, and following alcohol consumption.

Tue Nov 01 00:00:00 EST 2005

Monocytes treated with human immunodeficiency virus Tat kill uninfected CD4(+) cells by a tumor necrosis factor-related apoptosis-induced ligand-mediated mechanism.

Journal of virology

Yang Y,Tikhonov I,Ruckwardt TJ,Djavani M,Zapata JC,Pauza CD,Salvato MS

16-5951 was used in Neutralization experiments to show that production of TRAIL by Tat-stimulated monocytes provides a mechanism by which HIV infection can destroy uninfected bystander cells.

Sun Jun 01 00:00:00 EDT 2003

生物信息学

蛋白别名: CD253; CD253 antigen; Protein TRAIL; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL protein; TRAIL/APO2L; Tumor necrosis factor ligand superfamily member 10

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基因别名: A330042I21Rik; AI448571; APO-2L; Ly81; TL2; Tnfsf10; Trail

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UniProt ID: (Mouse) P50592

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Entrez Gene ID: (Mouse) 22035

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功能

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

参与通路

apoptotic process immune response positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of release of cytochrome c from mitochondria positive regulation of extrinsic apoptotic signaling pathway