Invitrogen

CD26 Monoclonal Antibody (H194-112 (H194-112.12.4.4.5)), PerCP-Cyanine5.5, eBioscience™

Name: CD26 Monoclonal Antibody (H194-112 (H194-112.12.4.4.5)), PerCP-Cyanine5.5, eBioscience\u2122
Brand: Invitrogen
SKU: 45-0261-82

2个已发表图片

13 篇参考文献

查看其他58个DPP4抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (2)

图: 1 / 3

CD26 Antibody (45-0261-82) in Flow

Staining of C57BL/6 thymocytes with 0.5 µg of Rat IgG2a kappa Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (blue histogram) or 0.5 µg of Anti-Mouse CD26 PerCP-Cyanine5-5 (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

产品信息

45-0261-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看13篇已发表文章 13篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

H194-112 (H194-112.12.4.4.5)

偶联物

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

激发/发射光谱

489/679 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1548738

产品详细信息

Description: The monoclonal antibody H194-112 recognizes mouse CD26, a thymocyte-activating molecule (THAM). CD26 is a surface expressed and soluble ectoenzyme (dipeptidyl peptidase IV) that cleaves dipeptides from the amino terminus of proteins. In particular CD26 has been shown to cleave CXCL12, CCL22, neuropeptide Y and glucagon. CD26 is involved in adhesion, apoptosis, disease regulation, and engraftment. Expression is regulated during thymic development at the messenger RNA level. Additionally, CD26 protein is found on thymocytes, a percentage of resting B cells, stimulated B cells, NK cells and a percentage of resting and activated T cells and other epithelial cell types. On human cells more CD26 protein is found on Th2 cells. Functional CD26 is expressed on Sca-1+c-Kit+lin- cells. This expression has been shown to correlate with mobilization of progenitor cells. Inhibition of CD26 activity through a variety of compounds enhances allogenic stem cell homing and engraftment.

This antibody is reported to have activating function.

Applications Reported: This H194-112 (H194-112.12.4.4.5) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H194-112 (H194-112.12.4.4.5) antibody has been tested by flow cytometric analysis of mouse splenocytes or thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family. CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity. CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts. Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。