Invitrogen

CD261 (DR4) Monoclonal Antibody (DJR1), Biotin, eBioscience™

CD261 (DR4) Antibody in Flow Cytometry (Flow)

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CD261 (DR4) Antibody (13-6644-82) in Flow

Staining of human DR4 transfected cells with Anti-Human CD261 (DR4) Biotin Streptavidin PE (Product # 12-4317-87). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD261 (DR4) Antibody in Western Blot (WB)

CD261 (DR4) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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产品信息

13-6644-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.06 µg/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

DJR1

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466889

产品详细信息

Description: The DJR1 monoclonal antibody reacts with human DR4, also known as TRAIL-R1. DR4 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR4 is expressed by a variety of human tumor cells and at very low level on human leukocytes.

Expression has also been found inside the cell on some cell lines (Zhang et al). This has been confirmed with intracellular staining of Jurkat cell line. Please contact tech@ebioscience.com for more information.

Applications Reported: The DJR1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The DJR1 antibody has been tested by flow cytometric analysis of human DR4-transfected cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking.

Cell death and differentiation

Fauster A,Rebsamen M,Willmann KL,César-Razquin A,Girardi E,Bigenzahn JW,Schischlik F,Scorzoni S,Bruckner M,Konecka J,Hörmann K,Heinz LX,Boztug K,Superti-Furga G

Published figure using CD261 (DR4) monoclonal antibody (Product # 13-6644-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Targeting the metabolic pathway of human colon cancer overcomes resistance to TRAIL-induced apoptosis.

Cell death discovery

Carr RM,Qiao G,Qin J,Jayaraman S,Prabhakar BS,Maker AV

13-6644 was used in Flow cytometry/Cell sorting to investigate mechanisms to alter glucose metabolism in order to selectively target the cancer cells and to overcome evasion of apoptosis.

Tue Sep 20 00:00:00 EDT 2016

Targeting the metabolic pathway of human colon cancer overcomes resistance to TRAIL-induced apoptosis.

Cell death discovery

Carr RM,Qiao G,Qin J,Jayaraman S,Prabhakar BS,Maker AV

13-6644 was used in Flow cytometry/Cell sorting to investigate mechanisms to alter glucose metabolism in order to selectively target the cancer cells and to overcome evasion of apoptosis.

Tue Sep 20 00:00:00 EDT 2016

TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids.

PloS one

Chandrasekaran S,Marshall JR,Messing JA,Hsu JW,King MR

Published figure using CD261 (DR4) monoclonal antibody (Product # 13-6644-82) in Flow Cytometry

Thu Dec 24 00:00:00 EST 2015

TRAIL-mediated apoptosis in breast cancer cells cultured as 3D spheroids.

PloS one

Chandrasekaran S,Marshall JR,Messing JA,Hsu JW,King MR

Published figure using CD261 (DR4) monoclonal antibody (Product # 13-6644-82) in Flow Cytometry

Thu Dec 24 00:00:00 EST 2015

Ubiquitination by the membrane-associated RING-CH-8 (MARCH-8) ligase controls steady-state cell surface expression of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) receptor 1.

The Journal of biological chemistry

van de Kooij B,Verbrugge I,de Vries E,Gijsen M,Montserrat V,Maas C,Neefjes J,Borst J

13-6644 was used in Flow cytometry/Cell sorting to investigate the functions and substrates of MARCH proteins.

Fri Mar 01 00:00:00 EST 2013

A whole-genome RNAi screen identifies an 8q22 gene cluster that inhibits death receptor-mediated apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Dompe N,Rivers CS,Li L,Cordes S,Schwickart M,Punnoose EA,Amler L,Seshagiri S,Tang J,Modrusan Z,Davis DP

Published figure using CD261 (DR4) monoclonal antibody (Product # 13-6644-82) in Flow Cytometry

Tue Oct 25 00:00:00 EDT 2011

Ionizing radiation modulates the TRAIL death-inducing signaling complex, allowing bypass of the mitochondrial apoptosis pathway.

Oncogene

Verbrugge I,de Vries E,Tait SW,Wissink EH,Walczak H,Verheij M,Borst J

13-6644 was used in Flow cytometry/Cell sorting to investigate the molecular mechanism underlying the response to combined modality treatment in p53-mutant Jurkat T leukemic cells overexpressing Bcl-2.

Thu Jan 24 00:00:00 EST 2008

Difference in cytotoxicity against hepatocellular carcinoma between liver and periphery natural killer cells in humans.

Hepatology (Baltimore, Md.)

Ishiyama K,Ohdan H,Ohira M,Mitsuta H,Arihiro K,Asahara T

Wed Feb 01 00:00:00 EST 2006

生物信息学

蛋白别名: CD261; cytotoxic TRAIL receptor; Death receptor 4; MGC9365; sCD261; soluble CD261; soluble TRAIL; sTRAIL; TNF-related apoptosis-inducing ligand receptor 1; TRAIL R1; TRAIL receptor 1; TRAIL-R1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a

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基因别名: APO2; CD261; DR4; TNFRSF10A; TRAILR-1; TRAILR1

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UniProt ID: (Human) O00220

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Entrez Gene ID: (Human) 8797

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功能

protease binding receptor activity tumor necrosis factor-activated receptor activity death receptor activity protein binding transcription factor binding TRAIL binding transmembrane signal receptor

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