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Invitrogen
CD270 (HVEM) Monoclonal Antibody (LH1), Functional Grade, eBioscience™
产品信息
16-5962-85
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Not Applicable
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
LH1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2637400
产品详细信息
Description: The LH1 monoclonal antibody reacts with mouse Herpes Virus Entry Mediator (HVEM, TR2), a member of the TNF-receptor superfamily. HVEM is found on most cell types, including T cells, B cells, monocytes, neutrophils and dendritic cells. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of HVEM was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. HVEM has also been demonstrated to be a unique ligand for BTLA (B and T lymphocyte attenuator). The conservation of the BTLA-HVEM interaction between mouse and human suggests that this system is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response. The LH1 antibody has been reported as a blocking antibody, interfering with the HVEM-LIGHT interaction but not the HVEM-BTLA interaction.
Applications Reported: This LH1 antibody has been reported for use in functional assays and flow cytometric analysis. (Fluorochrome-conjugated LH1 is recommended for use in flow cytometry).
Applications Tested: This LH1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
TNFRSF14 is a member of the TNF-receptor superfamily. TNFRSF14 was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to TNFRSF14 has been shown to be part of the viral entry mechanism. The cytoplasmic region of TNFRSF14 was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. Activation of the signal transduction pathway involving TNFRSF14 in T cells stimulates T cell proliferation and cytokine production, leading to inflammation and enhanced CTL-mediated tumor immunity, suggesting that these proteins may be useful as potential targets for controlling cellular immune responses. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these TNFRSF14 variants have not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD270; herpes virus entry mediator; Herpes virus entry mediator A; Herpesvirus entry mediator A; HGNC:11912; RP3-395M20.6; sCD2710; TR2; tumo; Tumor necrosis factor receptor superfamily member 14; Tumor necrosis factor receptor-like 2
基因别名: Atar; HveA; Hvem; Tnfrs14; Tnfrsf14
UniProt ID: (Mouse) Q80WM9
Entrez Gene ID: (Mouse) 230979
positive regulation of cytokine secretion involved in immune response
negative regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of T cell migration
