Invitrogen

CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), Alexa Fluor™ 488, eBioscience™

CD271 (NGF Receptor) Antibody in Flow Cytometry (Flow)

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CD271 (NGF Receptor) Antibody (53-9400-42) in Flow

Staining of 2-day Ibuprofen-stimulated HEK-293T cells with Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-42) (blue histogram) or CD271 (NGF Receptor) Monoclonal Antibody, Alexa Fluor 488 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

CD271 (NGF Receptor) Antibody in Immunohistochemistry (IHC)

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产品信息

53-9400-42

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

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产品规格

种属反应

Dog, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ME20.4

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2802341

产品详细信息

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.

The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.

Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy.

Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

靶标信息

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

In mouse chronic pancreatitis CD25⁺FOXP3⁺ regulatory T cells control pancreatic fibrosis by suppression of the type 2 immune response.

Nature communications

Glaubitz J,Wilden A,Golchert J,Homuth G,Völker U,Bröker BM,Thiele T,Lerch MM,Mayerle J,Aghdassi AA,Weiss FU,Sendler M

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 53-9400-42) in Flow Cytometry

Wed Aug 03 00:00:00 EDT 2022

Synovial membrane-derived mesenchymal progenitor cells from osteoarthritic joints in dogs possess lower chondrogenic-, and higher osteogenic capacity compared to normal joints.

Stem cell research & therapy

Teunissen M,Ahrens NS,Snel L,Narcisi R,Kamali SA,van Osch GJVM,Meij BP,Mastbergen SC,Sivasubramaniyan K,Tryfonidou MA

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 53-9400-42) in Immunohistochemistry

Mon Sep 05 00:00:00 EDT 2022

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD271 (NGF Receptor) monoclonal antibody (Product # 53-9400-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

生物信息学

蛋白别名: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 neurotrophin receptor; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16

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基因别名: CD271; Gp80-LNGFR; LNGFR; NGFR; NGR; p75; p75(NTR); p75NGFR; p75NTR; RNNGFRR; TNFRSF16

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UniProt ID: (Human) P08138, (Rat) P07174, (Mouse) Q9Z0W1