Invitrogen

CD278 (ICOS) Monoclonal Antibody (ISA-3), Super Bright™ 436, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (62-9948-42) in Flow

Staining of unstimulated (left) or Anti-Human CD3 and CD28 Functional Grade Purified (Product # 16-0037-81 and Product # 16-0289-81)-stimulated (right) normal human peripheral blood cells with Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (blue histogram) or Anti-Human CD278 (ICOS) Super Bright 436 (purple histogram). Total viable cells, as determined by 7-AAD Viability Staining Solution (Product # 00-6993-50), were used for analysis.

CD278 (ICOS) Antibody in Neutralization (Neu)

CD278 (ICOS) Antibody in T-Cell Activation (TCA)

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产品信息

62-9948-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看10篇已发表文章 10篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

T细胞活化 (TCA)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ISA-3

偶联物

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

激发/发射光谱

413/431 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2637393

产品详细信息

Description: The ISA-3 monoclonal antibody reacts with human CD278 also known as Inducible Costimulatory molecule (ICOS), H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (Product # 14-9949-82) , was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use Product # 13-9948-82 or Product # 12-9948-42 rather than Product # 11-9948-42 .

Applications Reported: This ISA-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Inducible Costimulator-C-X-C Motif Chemokine Receptor 3 Signaling is Involved in Chronic Obstructive Pulmonary Disease Pathogenesis.

International journal of chronic obstructive pulmonary disease

Li DY,Chen L,Miao SY,Zhou M,Wu JH,Sun SW,Liu LL,Qi C,Xiong XZ

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Neutralization

Tue Aug 23 00:00:00 EDT 2022

Functional SARS-CoV-2-Specific Immune Memory Persists after Mild COVID-19.

Cell

Rodda LB,Netland J,Shehata L,Pruner KB,Morawski PA,Thouvenel CD,Takehara KK,Eggenberger J,Hemann EA,Waterman HR,Fahning ML,Chen Y,Hale M,Rathe J,Stokes C,Wrenn S,Fiala B,Carter L,Hamerman JA,King NP,Gale M,Campbell DJ,Rawlings DJ,Pepper M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2021

Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.

eLife

Hill DL,Whyte CE,Innocentin S,Lee JL,Dooley J,Wang J,James EA,Lee JC,Kwok WW,Zand MS,Liston A,Carr EJ,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Changes in expression of PD-L1 on peripheral T cells in patients with melanoma and lung cancer treated with PD-1 inhibitors.

Scientific reports

Dart SJ,Cook AM,Millward MJ,McDonnell AM,Chin WL,Hakeem MU,Meniawy TM,Bowyer SE

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade.

Nature communications

Geuijen C,Tacken P,Wang LC,Klooster R,van Loo PF,Zhou J,Mondal A,Liu YB,Kramer A,Condamine T,Volgina A,Hendriks LJA,van der Maaden H,Rovers E,Engels S,Fransen F,den Blanken-Smit R,Zondag-van der Zande V,Basmeleh A,Bartelink W,Kulkarni A,Marissen W,Huang CY,Hall L,Harvey S,Kim S,Martinez M,O'Brien S,Moon E,Albelda S,Kanellopoulou C,Stewart S,Nastri H,Bakker ABH,Scherle P,Logtenberg T,Hollis G,de Kruif J,Huber R,Mayes PA,Throsby M

62-9948-42 was used in Flow Cytometry to describe a human CD137xPD-L1 bispecific antibody, MCLA-145, identified through functional screening of agonist- and immune checkpoint inhibitor arm combinations.

Wed Jul 21 00:00:00 EDT 2021

IL-21-Deficient T Follicular Helper Cells Support B Cell Responses Through IL-27 in Patients With Chronic Hepatitis B.

Frontiers in immunology

Khanam A,Ayithan N,Tang L,Poonia B,Kottilil S

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Wed Jun 16 00:00:00 EDT 2021

The adjuvant GLA-SE promotes human Tfh cell expansion and emergence of public TCRβ clonotypes.

The Journal of experimental medicine

Hill DL,Pierson W,Bolland DJ,Mkindi C,Carr EJ,Wang J,Houard S,Wingett SW,Audran R,Wallin EF,Jongo SA,Kamaka K,Zand M,Spertini F,Daubenberger C,Corcoran AE,Linterman MA

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Mon Aug 05 00:00:00 EDT 2019

Distribution of circulating follicular helper T cells and expression of interleukin-21 and chemokine C-X-C ligand 13 in gastric cancer.

Oncology letters

Meng X,Yu X,Dong Q,Xu X,Li J,Xu Q,Ma J,Zhou C

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Dysregulated ICOS⁺ proinflammatory and suppressive regulatory T cells in patients with rheumatoid arthritis.

Experimental and therapeutic medicine

Wang HX,Kang X,Chu S,Li H,Li X,Yin X,Qiu YR,Lai W

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Fri Jun 02 00:00:00 EDT 2017

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Polyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS.

Oncoimmunology

Franzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in T-Cell Activation

Sun May 01 00:00:00 EDT 2016

Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.

Oncoimmunology

Pedroza-Gonzalez A,Zhou G,Vargas-Mendez E,Boor PP,Mancham S,Verhoef C,Polak WG,Grünhagen D,Pan Q,Janssen H,Garcia-Romo GS,Biermann K,Tjwa ET,IJzermans JN,Kwekkeboom J,Sprengers D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 62-9948-42) in T-Cell Activation

Mon Jun 01 00:00:00 EDT 2015

生物信息学

蛋白别名: Activation-inducible lymphocyte immunomediatory molecule; CD278; inducible costimulator; inducible T-cell co-stimulator; Inducible T-cell costimulator

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基因别名: AILIM; CD278; CVID1; ICOS

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UniProt ID: (Human) Q9Y6W8

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Entrez Gene ID: (Human) 29851

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参与通路

T cell tolerance induction immune response single organismal cell-cell adhesion T cell costimulation

CD278 (ICOS) Monoclonal Antibody (ISA-3), Super Bright™ 436, eBioscience™

CD278 (ICOS) Antibody (62-9948-42) in Flow

产品信息

62-9948-42

流式细胞分析 (Flow)

中和实验 (Neu)

T细胞活化 (TCA)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

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