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Invitrogen

CD28 Recombinant Human Monoclonal Antibody (CD28.2)

Name: CD28 Recombinant Human Monoclonal Antibody (CD28.2)
Brand: Invitrogen
SKU: 740009H
Price: 5999.0 CNY

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查看其他136个CD28抗体

说明书

实验方案

技术支持

货号 740009H

CNY 5,999.00

下单后预计有货时间 24-Apr-2025

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说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

Application

图: 1 / 3

CD28 Antibody (740009H) in Flow

Jurkat cells were stained with 0.25 µg of CD28 Recombinant Human Monoclonal Antibody (CD28.2) (Product # 740009H), followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A-11013, 1:500) (pink histogram) or left unstained (blue histogram). Viable cells were used for analysis, as determined by LIVE/DEAD™ Fixable Viability Dye eFluor™ 780 (Product # 65-0865-14). The flow cytometry data was acquired using Attune™ NxT Flow Cytometer (Product # A29004).

Product Image Gallery

CD28 Antibody — ELISA based CD28:B7-1 Binding Inhibitor Screening Assay shows that the binding of Human CD28 to Human B7-1 (CD80) was inhibited by CD28 Recombinant Human Monoclonal Antibody (CD28.2) (Product # 740009H). Recombinant Human CD28 was coated at a concentration of 0.5 µg/mL onto a plate. This was pre-incubated with CD28 Recombinant Human Monoclonal Antibody (CD28.2) (Product # 740009H) at a concentration range of 100 to 10,000 ng/mL and binding signal of biotinylated Human B7-1 was detected using Streptavidin-HRP conjugate. The plate was developed using TMB stabilized chromogen solution (Product # SB02) and read at 450 nm with Thermo Scientific™ Varioskan™ LUX multimode microplate reader (Product # VLBLATD2). X axis represents antibody concentrations and Y axis represents percent binding signal of B7-1 to Human CD28. Dotted line represents 50% inhibition. {Neu}

产品信息

740009H

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.06-0.25 µg/test

酶联免疫吸附实验 (ELISA)

1:100-1:100,000

中和实验 (Neu)

1:100-1:100,000

产品规格

种属反应

Human

宿主/亚型

Human / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

CD28.2

偶联物

Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Research Use Only (RUO) human recombinant monoclonal antibodies are produced using in vitro expression systems and ideal to use as controls for development of assays from human samples. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

This product is a humanized format of a mouse monoclonal antibody.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD28; CD28 antigen; MGC138290; sCD28; soluble CD28; T-cell-specific surface glycoprotein CD28; T44; TP44

基因别名: CD28; Tp44

UniProt ID: (Human) P10747

Entrez Gene ID: (Human) 940

功能

protease binding SH3/SH2 adaptor activity protein binding coreceptor activity protein kinase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity immunoglobulin receptor superfamily

参与通路