Invitrogen

CD281 (TLR1) Monoclonal Antibody (eBioTR23 (TR23)), PE, eBioscience™

CD281 (TLR1) Antibody in Flow Cytometry (Flow)

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CD281 (TLR1) Antibody (12-9011-80) in Flow

Staining of A20 cells with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD281 (TLR1) PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-9011-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看8篇已发表文章 8篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioTR23 (TR23)

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_657857

产品详细信息

Description: The eBioTR23 monoclonal antibody reacts with mouse Toll-like receptor 1 (TLR1, CD281). TLR1 is an approximately 98 kDa protein belonging to the Toll-like receptor family, and is closely-related to mouse TLR6 in both structure and function. Through the formation of a complex with TLR2, TLR1 is able to bind bacterially-derived triacyl lipopeptides, as well as soluble factors released from Neisseria meningitidis. TLR1 is expressed by macrophages and macrophages in TLR1-deficient mice display impaired production of inflammatory cytokines upon stimulation with triacyl lipopeptides and lipoproteins from mycobacteria.

Applications Reported: This eBioTR23 (TR23) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioTR23 (TR23) antibody has been tested by flow cytometric analysis of A20 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR1 protein is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and co-expression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several tri-acylated lipopeptides. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for the TLR1 gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.

Journal of neuroscience research

Huang Y,Zhang B,Haneke H,Haage V,Lubas M,Yuan Y,Xia P,Motta E,Nanvuma C,Dzaye O,Hu F,Kettenmann H

12-9011-80 was used in Flow Cytometry to provide evidence that glial cell line-derived neurotrophic factor is capable to induce microglial MMP9/14 production and thus promotes glioma growth.

Thu Apr 01 00:00:00 EDT 2021

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

12-9011-80 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 EST 2021

Myeloid differentiation factor 88 signaling in donor T cells accelerates graft-versus-host disease.

Haematologica

Matsuoka S,Hashimoto D,Kadowaki M,Ohigashi H,Hayase E,Yokoyama E,Hasegawa Y,Tateno T,Chen X,Aoyama K,Oka H,Onozawa M,Takeda K,Akashi K,Teshima T

12-9011 was used in Flow cytometry/Cell sorting to identify that donor T-cell MyD88/interleukin-1-receptor associated kinase 4 pathway as a potential novel therapeutic target against graft-versus host disease after allo-SCT.

Wed Jan 01 00:00:00 EST 2020

Peptidoglycan-treated tumor antigen-pulsed dendritic cells impart complete resistance against tumor rechallenge.

Clinical and experimental immunology

Patidar A,Selvaraj S,Chauhan P,Guzman CA,Ebensen T,Sarkar A,Chattopadhyay D,Saha B

Published figure using CD281 (TLR1) monoclonal antibody (Product # 12-9011-80) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Increased White Matter Inflammation in Aging- and Alzheimer's Disease Brain.

Frontiers in molecular neuroscience

Raj D,Yin Z,Breur M,Doorduin J,Holtman IR,Olah M,Mantingh-Otter IJ,Van Dam D,De Deyn PP,den Dunnen W,Eggen BJL,Amor S,Boddeke E

12-9011 was used in Flow cytometry/Cell sorting to indicate that microglia-induced neuroinflammation is predominant in the white matter of aging mice and humans as well as in EOAD brains.