Invitrogen

CD289 (TLR9) Monoclonal Antibody (eB72-1665), PE, eBioscience™

CD289 (TLR9) Antibody in Flow Cytometry (Flow)

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CD289 (TLR9) Antibody (12-9099-82) in Flow

Staining of non-transfected (left) and human CD289-transfected (right) HEK 293T cells with 1.0 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 1.0 µg of Anti-Human CD289 (TLR9) PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-9099-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Human

已发表种属

Human, Pig

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eB72-1665

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466239

产品详细信息

Description: eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy (Nat Immunol. 5:190). Human Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested: This eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients.

BMC research notes

Deeba E,Lambrianides A,Pantzaris M,Krashias G,Christodoulou C

12-9099-82 was used in Flow Cytometry to study toll-like receptor (TLR) expression in the natural killer cells of patients with multiple sclerosis.

Tue Sep 29 00:00:00 EDT 2020

Interaction between Toll-Like Receptor 9-CpG Oligodeoxynucleotides and Hepatitis B Virus Virions Leads to Entry Inhibition in Hepatocytes and Reduction of Alpha Interferon Production by Plasmacytoid Dendritic Cells.

Antimicrobial agents and chemotherapy

Aillot L,Bonnin M,Ait-Goughoulte M,Bendriss-Vermare N,Maadadi S,Dimier L,Subic M,Scholtes C,Najera I,Zoulim F,Lucifora J,Durantel D

Sun Apr 01 00:00:00 EDT 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD289 (TLR9) monoclonal antibody (Product # 12-9099-82) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis.

Oncology letters

Zhang F,Wang D,Chen F

12-9099-82 was used in flow cytometry to investigate the correlation between the expression of Toll-like receptor-9 (TLR-9) and cell proliferation and apoptosis in hypoxic nasopharyngeal carcinoma cells.

Fri Dec 01 00:00:00 EST 2017

Toll-like receptor 9 signaling regulates tissue factor and tissue factor pathway inhibitor expression in human endothelial cells and coagulation in mice.

Critical care medicine

El Kebir D,Damlaj A,Makhezer N,Filep JG

12-9099 was used in Flow cytometry/Cell sorting to investigate the effects of CpG DNA on the expression and activity of tissue factor in human coronary artery endothelial cells and on coagulation in mice.

Mon Jun 01 00:00:00 EDT 2015

Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics.

Viral immunology

Liu F,Li G,Wen K,Bui T,Cao D,Zhang Y,Yuan L

12-9099 was used in Flow cytometry/Cell sorting to establish a novel in-vitro model of rotavirus infection and probiotic treatment.

Thu Apr 01 00:00:00 EDT 2010

Bacterial DNA activates endothelial cells and promotes neutrophil adherence through TLR9 signaling.

Journal of immunology (Baltimore, Md. : 1950)

El Kebir D,József L,Pan W,Wang L,Filep JG

12-9099 was used in Flow cytometry/Cell sorting to demonstrate that bacterial DNA promotes beta(2) integrin and E-selectin-mediated HUVEC-neutrophil adherence via TLR9 signalling.

Wed Apr 01 00:00:00 EDT 2009

Toll-like receptor and innate cytokine responses induced by lactobacilli colonization and human rotavirus infection in gnotobiotic pigs.

Veterinary immunology and immunopathology

Wen K,Azevedo MS,Gonzalez A,Zhang W,Saif LJ,Li G,Yousef A,Yuan L

12-9099 was used in Flow cytometry/Cell sorting to evaluate TLR2-, TLR3- and TLR9-expressing antigen presenting cell responses in the spleens and blood of gnotobiotic pigs.

Sun Feb 15 00:00:00 EST 2009

Human Langerhans cells selectively activated via Toll-like receptor 2 agonists acquire migratory and CD4+T cell stimulatory capacity.

Journal of leukocyte biology

Peiser M,Koeck J,Kirschning CJ,Wittig B,Wanner R

12-9099 was used in Flow cytometry/Cell sorting to analyse the Toll-like receptor (TLR) phenotype of epidermal Langerhans cells treated with various TLR agonists.

Thu May 01 00:00:00 EDT 2008

Local activation of the innate immune system in Buruli ulcer lesions.

The Journal of investigative dermatology

Peduzzi E,Groeper C,Schütte D,Zajac P,Rondini S,Mensah-Quainoo E,Spagnoli GC,Pluschke G,Daubenberger CA

Thu Mar 01 00:00:00 EST 2007

Activation of TLR-9 induces IL-8 secretion through peroxynitrite signaling in human neutrophils.

Journal of immunology (Baltimore, Md. : 1950)

József L,Khreiss T,El Kebir D,Filep JG

Sun Jan 15 00:00:00 EST 2006

Expansion of toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process.

Arthritis and rheumatism

Papadimitraki ED,Choulaki C,Koutala E,Bertsias G,Tsatsanis C,Gergianaki I,Raptopoulou A,Kritikos HD,Mamalaki C,Sidiropoulos P,Boumpas DT

12-9099 was used in Flow cytometry/Cell sorting to examine the expression and function of various TLRs in subpopulations of peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Wed Nov 01 00:00:00 EST 2006

TLR9- and FcepsilonRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression.

Journal of immunology (Baltimore, Md. : 1950)

Schroeder JT,Bieneman AP,Xiao H,Chichester KL,Vasagar K,Saini S,Liu MC

Tue Nov 01 00:00:00 EST 2005

TLR9- and FcepsilonRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression.

Journal of immunology (Baltimore, Md. : 1950)

Schroeder JT,Bieneman AP,Xiao H,Chichester KL,Vasagar K,Saini S,Liu MC

Tue Nov 01 00:00:00 EST 2005

Expression of toll-like receptors on B lymphocytes.

Cellular immunology

Dasari P,Nicholson IC,Hodge G,Dandie GW,Zola H

Thu Feb 16 00:00:00 EST 2006

Toll-like receptor 9 can be expressed at the cell surface of distinct populations of tonsils and human peripheral blood mononuclear cells.

Infection and immunity

Eaton-Bassiri A,Dillon SB,Cunningham M,Rycyzyn MA,Mills J,Sarisky RT,Mbow ML

12-9099 was used in Flow cytometry/Cell sorting to validate human TLR9 expression at the surface of primary cells, in addition to the previously described intracellular localization.

Wed Dec 01 00:00:00 EST 2004

生物信息学

蛋白别名: CD289; Toll-like receptor 9

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基因别名: CD289; TLR9; UNQ5798/PRO19605

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UniProt ID: (Human) Q9NR96

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Entrez Gene ID: (Human) 54106

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功能

transmembrane signaling receptor activity interleukin-1 receptor binding 1-phosphatidylinositol-3-kinase activity siRNA binding

参与通路

CD289 (TLR9) Monoclonal Antibody (eB72-1665), PE, eBioscience™

CD289 (TLR9) Antibody (12-9099-82) in Flow

产品信息

12-9099-82

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

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