Invitrogen

CD289 (TLR9) Monoclonal Antibody (eB72-1665), eBioscience™

CD289 (TLR9) Antibody in Flow Cytometry (Flow)

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CD289 (TLR9) Antibody (14-9099-82) in Flow

HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC (Product # 11-4811-85) alone (thin line) or with 1 µg of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).

CD289 (TLR9) Antibody in Western Blot (WB)

CD289 (TLR9) Antibody in Western Blot, Immunoprecipitation (WB, IP)

CD289 (TLR9) Antibody in Western Blot, Flow Cytometry (WB, Flow)

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产品信息

14-9099-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

Assay-Dependent

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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免疫沉淀 (IP)

Assay-Dependent

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抑制试剂 (IA)

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产品规格

种属反应

Human

已发表种属

Hamster, Human

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

eB72-1665

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_468575

产品详细信息

Description: eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy (Nat Immunol. 5:190). Human Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Fluorochrome conjugated eB72-1665 is recommended for use in intracellular flow cytometry.) Preliminary in vitro blocking assays suggested that eB72-1665 may not be effective in blocking of CpG binding. Please contact us for further information.

Applications Tested: The eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

TLR9 expression in chronic lymphocytic leukemia identifies a promigratory subpopulation and novel therapeutic target.

Blood

Kennedy E,Coulter E,Halliwell E,Profitos-Peleja N,Walsby E,Clark B,Phillips EH,Burley TA,Mitchell S,Devereux S,Fegan CD,Jones CI,Johnston R,Chevassut T,Schulz R,Seiffert M,Agathanggelou A,Oldreive C,Davies N,Stankovic T,Liloglou T,Pepper C,Pepper AGS

14-9099-82 was used in Inhibition Assays to explore the molecular signalling mechanisms of chronic lymphocytic leukaemia to help improve current treatment options.

Thu Jun 03 00:00:00 EDT 2021

Human CD40 ligand-expressing type 3 innate lymphoid cells induce IL-10-producing immature transitional regulatory B cells.

The Journal of allergy and clinical immunology

Komlósi ZI,Kovács N,van de Veen W,Kirsch AI,Fahrner HB,Wawrzyniak M,Rebane A,Stanic B,Palomares O,Rückert B,Menz G,Akdis M,Losonczy G,Akdis CA

14-9099 was used in Flow cytometry/Cell sorting to investigate the ILC3-B-cell interaction that probably takes place in human tonsils.

Sun Jul 01 00:00:00 EDT 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Flow Cytometry

Tue Jan 02 00:00:00 EST 2018

ICOS⁺ Foxp3⁺ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.

International journal of cancer

Nagase H,Takeoka T,Urakawa S,Morimoto-Okazawa A,Kawashima A,Iwahori K,Takiguchi S,Nishikawa H,Sato E,Sakaguchi S,Mori M,Doki Y,Wada H

14909982 was used in flow cytometry to establish the T Cell receptor-inducible costimulator as a promising target for direct T regulatory cell-targeting therapeutic agents for gastric cancer

Wed Feb 01 00:00:00 EST 2017

Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis.

Oncology letters

Zhang F,Wang D,Chen F

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Western Blot

Fri Dec 01 00:00:00 EST 2017

Lactoferrin suppresses the Epstein-Barr virus-induced inflammatory response by interfering with pattern recognition of TLR2 and TLR9.

Laboratory investigation; a journal of technical methods and pathology

Zheng Y,Qin Z,Ye Q,Chen P,Wang Z,Yan Q,Luo Z,Liu X,Zhou Y,Xiong W,Ma J,Li G

14-9099 was used in Immunoprecipitation to report that lactoferrin reduces synthesis of IL-8 and MCP-1 induced by Epstein-Barr virus, by suppressing NF-κB activity.

Sat Nov 01 00:00:00 EDT 2014

A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.

Retrovirology

Babu R,Brown A

14-9099 was used in Flow cytometry/Cell sorting to determine whether macrophage surface marker phenotype in non-polarised cultures is altered by HIV infection independently of host genetic background.

Mon Dec 16 00:00:00 EST 2013

Double-stranded RNA-mediated TLR3 activation is enhanced by CD14.

Immunity

Lee HK,Dunzendorfer S,Soldau K,Tobias PS

14-9099 was used in Immunocytochemistry-immunoflourescence to study the effect of CD14 on double-stranded RNA-mediated Toll-like receptor 3 activation.