Invitrogen

CD3/CD4/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor™ 710, eBioscience™

CD3/CD4/CD8a Antibody in Flow Cytometry (Flow)

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CD3/CD4/CD8a Antibody (22-0306-71) in Flow

Staining of normal human peripheral blood cells with eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor® 710, CD8a APC. Red blood cells were lysed using the 10X RBC Lysis Buffer (Multi-Species) (Product # 00-4300-54). Cells in the lymphocyte gate were used for analysis.

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产品信息

22-0306-71

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

20 µL/test

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产品规格

种属反应

Human

已发表种属

Human, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Cocktail

类型

Antibody

克隆号

SK3, SK7, SK1

偶联物

APC , FITC , PerCP-eFluor™ 710 APC , FITC , PerCP-eFluor™ 710 APC , FITC , PerCP-eFluor™ 710

形式

Liquid

浓度

20 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10671078

产品详细信息

Description: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a.

This cocktail contains the following antibodies:

Anti-Human CD4 (SK3) FITC

Anti-Human CD3 (SK7) PerCP-eFluor® 710

Anti-Human CD8a (SK1) APC

Applications Reported: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor® 710, CD8a APC has been reported for use in flow cytometric analysis.

Applications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor® 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor® 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

靶标信息

CD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Recurrent visceral leishmaniasis relapses in HIV co-infected patients are characterized by less efficient immune responses and higher parasite load.

iScience

Takele Y,Mulaw T,Adem E,Womersley R,Kaforou M,Franssen SU,Levin M,Taylor GP,Müller I,Cotton JA,Kropf P

22-0306-71 was used in Flow cytometry/Cell sorting to show that the relapse-free survival in patients with recurrent VL/HIV was shorter, that they had higher parasite load, lower weight gain, and lower recovery of all blood cell lineages.

Fri Feb 17 00:00:00 EST 2023

Immunological factors, but not clinical features, predict visceral leishmaniasis relapse in patients co-infected with HIV.

Cell reports. Medicine

Takele Y,Mulaw T,Adem E,Shaw CJ,Franssen SU,Womersley R,Kaforou M,Taylor GP,Levin M,Müller I,Cotton JA,Kropf P

22-0306-71 was used in Flow Cytometry to identify three immunological markers associated with visceral leishmaniasis relapse in VL/HIV patients.

Tue Jan 18 00:00:00 EST 2022

Mucosal integrin α4β7 blockade fails to reduce the seeding and size of viral reservoirs in SIV-infected rhesus macaques.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ziani W,Shao J,Fang A,Connolly PJ,Wang X,Veazey RS,Xu H

22-0306-71 was used in Flow Cytometry to investigate the efficacy of prophylactic integrin blockade on viral infection, as well as dissemination and seeding of viral reservoirs in systemic and lymphoid compartments post-SIV inoculation.

Mon Feb 01 00:00:00 EST 2021

Differences in Maturation Status and Immune Phenotypes of Circulating Helios⁺ and Helios^- Tregs and Their Disrupted Correlations With Monocyte Subsets in Autoantibody-Positive T1D Individuals.

Frontiers in immunology

Zhang Y,Zhang J,Shi Y,Shen M,Lv H,Chen S,Feng Y,Chen H,Xu X,Yang T,Xu K

22-0306-71 was used in Flow Cytometry to explore the immunological changes in circulating Helios+ and Helios- regulator T-cells in type 1 diabetes.

Wed Sep 15 00:00:00 EDT 2021

生物信息学

蛋白别名: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 epsilon; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; FLJ18683; Leu-2; Leu2 T-lymphocyte antigen; OKT3, delta chain; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen; TCR zeta chain

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基因别名: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; CD4; CD4mut; CD8; CD8A; CD8B; CD8B1; IMD17; IMD18; IMD19; IMD25; LEU2; LY3; LYT3; MAL; p32; P37; T3D; T3E; T3G; T3Z; TCRE; TCRZ