Invitrogen

CD314 (NKG2D) Monoclonal Antibody (1D11), Biotin, eBioscience™

CD314 (NKG2D) Antibody in Flow Cytometry (Flow)

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CD314 (NKG2D) Antibody (13-5878-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0089-42) and 0.06 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (left) or 0.06 µg of Anti-Human CD314 (NKG2D) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). CD3+ cells in the lymphocyte gate were used for analysis.

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产品信息

13-5878-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1D11

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466735

产品详细信息

Description: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.

Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular C terminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients.

Genes & diseases

Zhang H,Huang C,Chen X,Li L,Liu S,Li Y,Zhang Y,Zeng Y,Hu L

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 13-5878-82) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

miR-20a regulates sensitivity of colorectal cancer cells to NK cells by targeting MICA.

Bioscience reports

Tang S,Fu H,Xu Q,Zhou Y

Published figure using CD314 (NKG2D) monoclonal antibody (Product # 13-5878-82) in Flow Cytometry

Wed Jul 31 00:00:00 UTC 2019

A chimeric receptor with NKG2D specificity enhances natural killer cell activation and killing of tumor cells.

Cancer research

Chang YH,Connolly J,Shimasaki N,Mimura K,Kono K,Campana D

13-5878 was used in Flow cytometry/Cell sorting to show the cytotoxic potential of NK cells against tumour subtypes could be markedly enhanced by expression of NKG2D-DAP10-CD3ζ receptors.

Fri Mar 15 00:00:00 UTC 2013

NKG2D receptor regulates human effector T-cell cytokine production.

Blood

Barber A,Sentman CL

13-5878 was used in Magnetic cell separation to reveal that human effector T cells alter their function depending on which innate receptors are engaged in conjunction with the TCR complex.

Thu Jun 16 00:00:00 UTC 2011

生物信息学

蛋白别名: CD314; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; natural killer cell receptor; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2-D-activating NK receptor

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基因别名: CD314; D12S2489E; KLR; KLRK1; NKG2-D; NKG2D

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UniProt ID: (Human) P26718

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Entrez Gene ID: (Human) 22914

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功能

receptor activity protein binding kinase binding carbohydrate binding MHC class Ib receptor activity MHC class I protein binding protein homodimerization activity

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