Invitrogen

CD34-T Polyclonal Antibody

CD34-T Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 2

CD34-T Antibody (PA5-142938) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of CD34-T in U2OS cells using CD34-T Polyclonal Antibody (Product # PA5-142938). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane, nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Product Image Gallery

产品信息

PA5-142938

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

免疫细胞化学 (ICC/IF)

10 µg/mL

流式细胞分析 (Flow)

10 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Peptide with sequence C-SPTGERLELEP.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2933580

产品详细信息

Additional Information: This antibody is expected to recognize reported isoform CD34-T (NP_001020280.1) only.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.

靶标信息

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]. This target recognizes CD34-T isoform.

仅用于科研。不用于诊断过程。未经明确授权不得转售。