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CD354 (TREM-1) Antibody (50-3541-82) in Flow
Staining of mouse peripheral blood cells with 0.06 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or 0.06 µg of Anti-Mouse CD354 (TREM-1) eFluor® 660 (purple histogram). Cells in the granulocyte gate were used for analysis.
产品信息
50-3541-82
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
TR3MBL1
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2574205
产品详细信息
Description: The TR3MBL1 monoclonal antibody recognizes mouse CD354 (also known as TREM-1), a 30-kDa glycosylated member of the Ig superfamily. CD354 is expressed on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, some macrophages, platelets, and megakaryocytes. Outside the immune system, CD354 is expressed on hepatocytes, osteoclasts, and microglia. CD354 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. CD354 plays a critical role in the innate immune response to bacterial and fungal infection by triggering the release of pro-inflammatory chemokines and cytokines. The cell surface receptor associates constitutively with the ITAM-containing adaptor protein DAP12 to activate the MAPK and NFkB signaling pathways. Finally, levels of the soluble receptor are increased in several inflammatory diseases, including sepsis, asthma, and inflammatory bowel disease, making this receptor a valuable diagnostic biomarker.
Applications Reported: This TR3MBL1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TR3MBL1 antibody has been tested by flow cytometric analysis of mouse peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
TREM1 (triggering receptor expressed on myeloid cells 1) is a receptor belonging to the Ig superfamily that amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. It is thought that TREM1 other related TREM proteins such as TREM2 act to modulate the inflammatory response. TREM1 has also been linked to inflammatory bowel disease resulting in enhanced production of TNF, IL-6, IL-8, and MCP-1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD354; sCD354; soluble CD354; soluble TREM 1; soluble TREM1; sTREM 1; sTREM1; TREM; TREM-1; triggering receptor expressed in monocytes 1; Triggering receptor expressed on myeloid cells; Triggering receptor expressed on myeloid cells 1
基因别名: Trem1
UniProt ID: (Mouse) Q9JKE2
Entrez Gene ID: (Mouse) 58217
positive regulation of defense response to virus by host
cytokine secretion involved in immune response
cell migration
neutrophil chemotaxis
cytokine metabolic process
chemokine metabolic process
neutrophil mediated killing of gram-negative bacterium
neutrophil extravasation
mitophagy in response to mitochondrial depolarization
