Invitrogen

CD363 (S1PR1) Monoclonal Antibody (SW4GYPP), eFluor™ 660, eBioscience™

CD363 (S1PR1) Antibody in Flow Cytometry (Flow)

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CD363 (S1PR1) Antibody (50-3639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (Product # 11-0047-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human CD363 (S1PR1) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

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产品信息

50-3639-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

查看7篇已发表文章 7篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SW4GYPP

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2574208

产品详细信息

Description: The SW4GYPP monoclonal antibody recognizes human CD363, also known as sphingosine-1-phosphate receptor 1 (S1PR1, S1P1), or endothelial differentiation G-protein coupled receptor 1 (EDG1). CD363 is a part of a family of five G-protein-coupled receptors that bind sphingosine-1-phosphate (S1P), a lysophospholipid mediator. It has been shown to be primarily expressed by lymphocytes but is also seen on endothelial cells, B cells, NK cells, monocytes/macrophages, neural progenitors, neurons, astrocytes, and oligodendrocytes. CD363 surface expression is dependent on the extracellular concentration of S1P and its signaling is important for lymphocyte egress from secondary lymphoid tissues and the thymus. CD363 plays a key role in many cellular processes like angiogenesis, vascular maturation, endothelial barrier function and trafficking of immune cells. Therapeutic CD363 modulators have been successful in the treatment of relapsing-remitting multiple sclerosis through sequestration of immune cells in secondary lymphoid organs and, therefore, away from sites of inflammation.

Applications Reported: This SW4GYPP antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SW4GYPP antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Heterozygous premature termination in zinc-finger domain of Krüppel-like factor 2 gene associates with dysregulated immunity.

Frontiers in immunology

Pernaa N,Keskitalo S,Chowdhury I,Nissinen A,Glumoff V,Keski-Filppula R,Junttila J,Eklund KK,Santaniemi W,Siitonen S,Seppänen MR,Vähäsalo P,Varjosalo M,Åström P,Hautala T

50-3639-42 was used in Flow Cytometry to conclude that KLF2 variant may be associated with familial immune dysregulation. Although the KLF2 deficient family members in our study suffered from lymphopenia, autoimmunity or malignancy, additional study cohorts are required to confirm our observations.

Tue Dec 06 00:00:00 EST 2022

In Vitro Characterization of Sphingosine 1-Phosphate Receptor 1 (S1P₁) Expression and Mediated Migration of Primary Human T and B Cells in the Context of Cenerimod, a Novel, Selective S1P₁ Receptor Modulator.

International journal of molecular sciences

Schlicher L,Kulig P,von Münchow A,Murphy MJ,Keller MP

50-3639-42 was used in Flow cytometry/Cell sorting to show that cenerimod effectively prevents T and B cell migration in a concentration-dependent manner.

Fri Jan 21 00:00:00 EST 2022

Resident memory T cells are a cellular reservoir for HIV in the cervical mucosa.

Nature communications

Cantero-Pérez J,Grau-Expósito J,Serra-Peinado C,Rosero DA,Luque-Ballesteros L,Astorga-Gamaza A,Castellví J,Sanhueza T,Tapia G,Lloveras B,Fernández MA,Prado JG,Solé-Sedeno JM,Tarrats A,Lecumberri C,Mañalich-Barrachina L,Centeno-Mediavilla C,Falcó V,Buzon MJ,Genescà M

50-3639-42 was used in Flow Cytometry to study cervical tissues from aviremic ART- treated HIV-1 infected women, to determine if T cells are a cellular reservoir for HIV.

Fri Oct 18 00:00:00 EDT 2019

Detection of HIV-1-specific gastrointestinal tissue resident CD8⁺ T-cells in chronic infection.

Mucosal immunology

Kiniry BE,Li S,Ganesh A,Hunt PW,Somsouk M,Skinner PJ,Deeks SG,Shacklett BL

Tue May 01 00:00:00 EDT 2018

Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.

International journal of molecular medicine

Ickrath P,Kleinsasser N,Ding X,Ginzkey C,Beyersdorf N,Hagen R,Kerkau T,Hackenberg S

50-3639 was used in Flow cytometry/Cell sorting to characterise CD69+ sphingosine-1-phosphate receptor 1 tissue resident memory T cells in the polyps of patients with chronic rhinosinusitis with nasal polyps.

Wed Aug 01 00:00:00 EDT 2018

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell reports

Simula L,Pacella I,Colamatteo A,Procaccini C,Cancila V,Bordi M,Tregnago C,Corrado M,Pigazzi M,Barnaba V,Tripodo C,Matarese G,Piconese S,Campello S

50-3639 was used in Flow cytometry/Cell sorting to describe the crucial role of the mitochondrial protein Drp1 in T cell development and regulation of lymphocyte immune-surveillance.

Tue Dec 11 00:00:00 EST 2018

Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.

Journal of immunology (Baltimore, Md. : 1950)

Cuff AO,Robertson FP,Stegmann KA,Pallett LJ,Maini MK,Davidson BR,Male V

50-3639 was used in Flow cytometry/Cell sorting to study the molecular signal that characterises an Eomeshi population of natural killer cells in the human liver that is not present in the blood.

Thu Dec 01 00:00:00 EST 2016

生物信息学

蛋白别名: CD antigen CD363; CD363; CD363 antigen; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; S1P receptor 1; S1P receptor Edg-1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

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基因别名: CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; S1P1; S1PR1

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UniProt ID: (Human) P21453

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Entrez Gene ID: (Human) 1901

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功能

G-protein coupled receptor binding G-protein coupled receptor activity sphingosine-1-phosphate receptor activity sphingolipid binding G-protein coupled receptor

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