Invitrogen

CD3e Monoclonal Antibody (eBio500A2 (500A2)), Alexa Fluor™ 700, eBioscience™

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CD3e Antibody (56-0033-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Anti-Mouse CD3e Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

CD3e Antibody in Immunohistochemistry (IHC)

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产品信息

56-0033-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.5 µg/test

查看11篇已发表文章 11篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

eBio500A2 (500A2)

偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_837094

产品详细信息

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.

Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

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56-0033-82 was used in Flow Cytometry to show that LdCen-/- containing neutrophils phagocytized by DC markedly influence the phenotype and antigen presenting capacity of DCs early on and thus play an immune-regulatory role in shaping vaccine induced host protective response.

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Park JG,Oladunni FS,Rohaim MA,Whittingham-Dowd J,Tollitt J,Hodges MDJ,Fathallah N,Assas MB,Alhazmi W,Almilaibary A,Iqbal M,Chang P,Escalona R,Shivanna V,Torrelles JB,Worthington JJ,Jackson-Jones LH,Martinez-Sobrido L,Munir M

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Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist.

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Glassman CR,Su L,Majri-Morrison SS,Winkelmann H,Mo F,Li P,Pérez-Cruz M,Ho PP,Koliesnik I,Nagy N,Hnizdilova T,Picton LK,Kovar M,Bollyky P,Steinman L,Meyer E,Piehler J,Leonard WJ,Garcia KC

56-0033 was used in Flow cytometry/Cell sorting to show that cytokine partial agonists can be used to calibrate intrinsic differences in response thresholds across responding cell types to narrow pleiotropic actions, which may be generalizable to other cytokine and growth factor systems.

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Published figure using CD3e monoclonal antibody (Product # 56-0033-82) in Flow Cytometry

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Development of a SFTSV DNA vaccine that confers complete protection against lethal infection in ferrets.

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Kim AR,Park J,Kim JH,Kwak JE,Cho Y,Lee H,Jeong M,Park SH,Shin EC

56-0033 was used in Flow cytometry/Cell sorting to evaluate the T cell immunogenicity of Herpes Zoster DNA vaccines.

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γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

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56-0033 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

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生物信息学

蛋白别名: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

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基因别名: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz