Invitrogen

CD4 Monoclonal Antibody (N1UG0), eFluor™ 570, eBioscience™

CD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD4 Antibody (41-2444-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 20 µg/mL of Anti-Human CD4 eFluor® 570. Nuclei are stained with DAPI.

CD4 Antibody in Immunohistochemistry (IHC)

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Product Image Gallery

产品信息

41-2444-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

20 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

20 µg/mL

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

N1UG0

偶联物

eFluor™ 570 eFluor™ 570 eFluor™ 570

激发/发射光谱

556/569 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573602

产品详细信息

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Applications Reported: This N1UG0 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This N1UG0 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low or high pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be titrated for opitmal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Intrahepatic activated leukocyte cell adhesion molecule induces CD6^highCD4⁺ T cell infiltration in autoimmune hepatitis.

Frontiers in immunology

Qian Q,Cui N,Huang B,Zhao Y,Liu Q,Hu M,Li B,Wang Q,Miao Q,You Z,Ma X,Tang R

Published figure using CD4 monoclonal antibody (Product # 41-2444-82) in Immunohistochemistry

Wed Sep 28 00:00:00 EDT 2022

Asymmetric T cell division of GAD65 specific naive T cells contribute to an early divergence in the differentiation fate into memory T cell subsets.

Immunology

Alampi G,Vignali D,Centorame I,Canu A,Cosorich I,Filoni J,Di Dedda C,Monti P

Published figure using CD4 monoclonal antibody (Product # 41-2444-82) in Immunocytochemistry

Tue Nov 01 00:00:00 EDT 2022

Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4⁺ Tissue Memory in situ T Cells in Primary Sjogren's Syndrome.

International journal of general medicine

Yang XX,Yang C,Wang L,Zhou YB,Yuan X,Xiang N,Wang YP,Li XM

Published figure using CD4 monoclonal antibody (Product # 41-2444-82) in Immunocytochemistry

Wed Apr 27 00:00:00 EDT 2022

Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4⁺ Tissue Memory in situ T Cells in Primary Sjogren's Syndrome.

International journal of general medicine

Yang XX,Yang C,Wang L,Zhou YB,Yuan X,Xiang N,Wang YP,Li XM

Published figure using CD4 monoclonal antibody (Product # 41-2444-82) in Immunocytochemistry

Wed Apr 27 00:00:00 EDT 2022

CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

The Journal of clinical investigation

Halkias J,Rackaityte E,Hillman SL,Aran D,Mendoza VF,Marshall LR,MacKenzie TC,Burt TD

Published figure using CD4 monoclonal antibody (Product # 41-2444-82) in Immunohistochemistry

Thu May 30 00:00:00 EDT 2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Scientific data

Rashid R,Gaglia G,Chen YA,Lin JR,Du Z,Maliga Z,Schapiro D,Yapp C,Muhlich J,Sokolov A,Sorger P,Santagata S

41-2444-82 was used in Immunohistochemistry (Paraffin) sorting to show that this dataset is a valuable resource for biological discovery of the immune system in normal and diseased states as well as for the development of multiplexed image analysis and viewing tools.