Invitrogen

CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor™ 561, eBioscience™

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CD4 Antibody (505-0049-42) in Flow

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, Alexa Fluor 532 (Product # 58-0038-42), CD19 Monoclonal Antibody, FITC (Product # 11-0199-42 ), CD8a Monoclonal Antibody, PE (Product # 12-0088-42), CD25 Monoclonal Antibody, PE-Cyanine7 (Product # 25-0259-42), CD56 (NCAM) Monoclonal Antibody, PE-eFluor 610 (Product # 61-0567-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 561 (Product # 606-4714-81) or CD4 Monoclonal Antibody, Alexa Fluor 561. Live cells as determined by Fixable Viability Dye eFluor 450 (... View More

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产品信息

505-0049-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

RPA-T4

偶联物

Alexa Fluor™ 561 Alexa Fluor™ 561 Alexa Fluor™ 561

激发/发射光谱

556/569 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2896187

产品详细信息

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This RPA-T4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Alexa Fluor 561 emits at 575 nm and is intended for use on spectral cytometers where it may be multiplexed with both PE and PE-eFluor 610.

Excitation: 558 nm; Emission: 575 nm; Laser: Yellow-Green Laser

靶标信息

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。