Invitrogen

CD41a Monoclonal Antibody (HIP8), eFluor™ 450, eBioscience™

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CD41a Antibody (48-0419-42) in Flow

Staining of human platelets with Anti-Human CD42b FITC (Product # 11-0429-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD41 eFluor® 450 (right). Total events were used for analysis.

CD41a Antibody in Immunocytochemistry (ICC/IF)

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产品信息

48-0419-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

5 µL (0.5 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

HIP8

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1944366

产品详细信息

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alpha IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation.

Applications Reported: This HIP8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD41a monoclonal antibody (Product # 48-0419-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

SARS-CoV-2 Receptors are Expressed on Human Platelets and the Effect of Aspirin on Clinical Outcomes in COVID-19 Patients.

Research square

Sahai A,Bhandari R,Koupenova M,Freedman JE,Godwin M,McIntyre T,Chung MK,Iskandar JP,Kamran H,Hariri E,Aggarwal A,Kalra A,Bartholomew JR,McCrae KR,Elbadawi A,Svensson LG,Kapadia S,Cameron SJ

Published figure using CD41a monoclonal antibody (Product # 48-0419-42) in Immunocytochemistry

Wed Dec 23 00:00:00 EST 2020

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using CD41a monoclonal antibody (Product # 48-0419-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Mitochondrial DNA in the tumour microenvironment activates neutrophils and is associated with worse outcomes in patients with advanced epithelial ovarian cancer.

British journal of cancer

Singel KL,Grzankowski KS,Khan ANMNH,Grimm MJ,D'Auria AC,Morrell K,Eng KH,Hylander B,Mayor PC,Emmons TR,Lénárt N,Fekete R,Környei Z,Muthukrishnan U,Gilthorpe JD,Urban CF,Itagaki K,Hauser CJ,Leifer C,Moysich KB,Odunsi K,Dénes Á,Segal BH

Published figure using CD41a monoclonal antibody (Product # 48-0419-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

The role of platelets in mediating a response to human influenza infection.

Nature communications

Koupenova M,Corkrey HA,Vitseva O,Manni G,Pang CJ,Clancy L,Yao C,Rade J,Levy D,Wang JP,Finberg RW,Kurt-Jones EA,Freedman JE

Published figure using CD41a monoclonal antibody (Product # 48-0419-42) in Immunocytochemistry

Tue Apr 16 00:00:00 EDT 2019

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

48-0419 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 EDT 2016

生物信息学

蛋白别名: alphaIIb protein; CD41; form 1; form 2; GPalpha IIb; GPIIb; Integrin alpha-IIb; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); platelet fibrinogen receptor, alpha subunit; platelet glycoprotein IIb of IIb/IIIa complex; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK; protein phosphatase 1, regulatory subunit 93

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基因别名: BDPLT16; BDPLT2; CD41; CD41B; GP2B; GPIIb; GT; GTA; HPA3; ITGA2B; ITGAB; PPP1R93

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UniProt ID: (Human) P08514

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Entrez Gene ID: (Human) 3674

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功能

protein binding identical protein binding metal ion binding extracellular matrix binding fibrinogen binding integrin

参与通路

platelet degranulation positive regulation of leukocyte migration cell adhesion cell-matrix adhesion integrin-mediated signaling pathway extracellular matrix organization platelet aggregation