Invitrogen

CD45RB Monoclonal Antibody (C363.16A), FITC, eBioscience™

CD45RB Antibody in Flow Cytometry (Flow)

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CD45RB Antibody (11-0455-82) in Flow

Staining of BALB/c splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control FITC (Product # 11-4321-42) (open histogram) or 0.25 µg of Anti-Mouse CD45RB FITC (filled histogram). Total viable cells were used for analysis.

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产品信息

11-0455-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

C363.16A

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_465064

产品详细信息

Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.

Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.

Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Cytosolic Nuclear Sensor Dhx9 Controls Medullary Thymic Epithelial Cell Differentiation by p53-Mediated Pathways.

Frontiers in immunology

Dong X,Zhang J,Zhang Q,Liang Z,Xu Y,Zhao Y,Zhang B

11-0455-82 was used in Flow cytometry/Cell sorting to reveal that Dhx9, a cytosolic nuclear sensor recognizes viral DNA or RNA, playing an important role in mTEC development and function in mice.

Tue Jun 21 00:00:00 EDT 2022

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

11-0455 was used in Flow cytometry/Cell sorting to conclude that Btnl proteins are required to select and to maintain the phenotypes of tissue-protective γδ IEL compartments, with combinatorially diverse heteromers having differential impacts on different IEL subsets.

Tue Jul 28 00:00:00 EDT 2020

Butyrophilin-like proteins display combinatorial diversity in selecting and maintaining signature intraepithelial γδ T cell compartments.

Nature communications

Jandke A,Melandri D,Monin L,Ushakov DS,Laing AG,Vantourout P,East P,Nitta T,Narita T,Takayanagi H,Feederle R,Hayday A

Tue Jul 28 00:00:00 EDT 2020

Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation.

The Journal of experimental medicine

Shin B,Kress RL,Kramer PA,Darley-Usmar VM,Bellis SL,Harrington LE

11-0455 was used in Flow cytometry/Cell sorting to indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties.

Mon Jul 02 00:00:00 EDT 2018

IL-12 and IL-4 activate a CD39-dependent intrinsic peripheral tolerance mechanism in CD8(+) T cells.

European journal of immunology

Noble A,Mehta H,Lovell A,Papaioannou E,Fairbanks L

11-0455 was used in Flow cytometry/Cell sorting to study the upregulation of CD39 on CD8(+) T cells and identify a novel pathway of peripheral tolerance.

Wed Jun 01 00:00:00 EDT 2016

Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii.

JCI insight

El Bissati K,Chentoufi AA,Krishack PA,Zhou Y,Woods S,Dubey JP,Vang L,Lykins J,Broderick KE,Mui E,Suzuki Y,Sa Q,Bi S,Cardona N,Verma SK,Fraczek L,Reardon CA,Sidney J,Alexander J,Sette A,Vedvick T,Fox C,Guderian JA,Reed S,Roberts CW,McLeod R

11-0455 was used in Flow cytometry/Cell sorting to develop multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant to protect against Toxoplasma gondii.

Thu Sep 22 00:00:00 EDT 2016

STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE.

Journal of neuroinflammation

McWilliams IL,Rajbhandari R,Nozell S,Benveniste E,Harrington LE

11-0455 was used in Flow cytometry/Cell sorting to establish the role of STAT4 in the regulation of cytokine production during experimental autoimmune encephalomyelitis pathogenesis.

Tue Jun 30 00:00:00 EDT 2015

Leishmania braziliensis infection induces dendritic cell activation, ISG15 transcription, and the generation of protective immune responses.

Journal of immunology (Baltimore, Md. : 1950)

Vargas-Inchaustegui DA,Xin L,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate whether Leishmania braziliensis infection efficiently triggers innate responses in dendritic cells and the subsequent adaptive immune responses for parasite clearance.

Sun Jun 01 00:00:00 EDT 2008

Effects of CXCL10 on dendritic cell and CD4+ T-cell functions during Leishmania amazonensis infection.

Infection and immunity

Vasquez RE,Xin L,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying enhanced immunity against L. amazonensis infection following CXC10 treatment, showing that treatment has an effect on dendritic cell and CD4+ T-cell functions.

Tue Jan 01 00:00:00 EST 2008

Role of interleukin-1beta in activating the CD11c(high) CD45RB- dendritic cell subset and priming Leishmania amazonensis-specific CD4+ T cells in vitro and in vivo.

Infection and immunity

Xin L,Li Y,Soong L

11-0455 was used in Flow cytometry/Cell sorting to investigate the mechanism by which the Leishmania amazonensis parasite modulates antigen-presenting cells and disrupts the immune system.

Mon Oct 01 00:00:00 EDT 2007

生物信息学

蛋白别名: CD45; L-CA; Leukocyte common antigen; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; T200

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基因别名: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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功能

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

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